TOP3A Polyklonaler Antikörper

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• KD/KO Validated

TOP3A Polyklonal Antikörper für IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

25

Rezensionen (1)

Kat-Nr. : 14525-1-AP

Synonyme

DNA topoisomerase 3 alpha, DNA topoisomerase III alpha, TOP3, TOP3A, topoisomerase (DNA) III alpha

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Galerie der Validierungsdaten

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  WB analysis of K-562 using 14525-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14525-1-AP (TOP3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 14525-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 14525-1-AP (TOP3A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 14525-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14525-1-AP (TOP3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 14525-1-AP

  IP Result of anti-TOP3A (IP:14525-1-AP, 4ug; Detection:14525-1-AP 1:500) with K-562 cells lysate 3600ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HL-60-Zellen
• Erfolgreiche IP: K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 7 publications below
• WB: See 22 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Produktinformation

14525-1-AP bindet in WB, IP, IHC, ELISA TOP3A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Krallenfrosch (Xenopus)
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TOP3A fusion protein Ag6010
• Vollständiger Name: topoisomerase (DNA) III alpha
• Berechnetes Molekulargewicht: 1001 aa, 112 kDa
• Beobachtetes Molekulargewicht: 100-110 kDa
• GenBank-Zugangsnummer: BC051748
• Gene symbol: TOP3A
• Gene ID (NCBI): 7156
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DNA topoisomerase 3-alpha (TOP3A) is an essential component of the RMI complex, a complex involves in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. It releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. This antibody is specific to react with the 110kd human TOP3A.

Publikationen

Species	Application	Title
human	WB	Mol Cell; TopBP1 interacts with BLM to maintain genome stability but is dispensable for preventing BLM degradation.; Authors - Andrew N Blackford
human	WB	Mol Cell; Topoisomerase 3α Is Required for Decatenation and Segregation of Human mtDNA.; Authors - Thomas J Nicholls ; KD Validated
		Sci Adv; The mitochondrial single-stranded DNA binding protein is essential for initiation of mtDNA replication.; Authors - Min Jiang
human	WB	Nat Commun; The Bloom syndrome complex senses RPA-coated single-stranded DNA to restart stalled replication forks.; Authors - Ann-Marie K Shorrocks
human	WB	Nucleic Acids Res; PMID: 30169847; Authors - Anu Hangas
human	WB,IHC	Nucleic Acids Res; Top3α is the replicative topoisomerase in mitochondrial DNA replication.; Authors - Anu Hangas ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Andrea (Verified Customer) (01-23-2023)

Strong and crisp band.

• Applications: Western Blot