TOP2A Polyklonaler Antikörper

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• KD/KO Validated

TOP2A Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 24641-1-AP

Synonyme

DNA topoisomerase 2 alpha, TOP II, TOP2, TOP2A, TOPII, TP2A

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Galerie der Validierungsdaten

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  WB analysis using 24641-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24641-1-AP (TOP2A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 24641-1-AP

  WB result of TOP2A antibody (24641-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOP2A transfected HepG2 cells.

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  WB analysis of HeLa using 24641-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24641-1-AP (TOP2A Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 24641-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24641-1-AP (TOP2A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 24641-1-AP

  IP Result of anti-TOP2A (IP:24641-1-AP, 4ug; Detection:24641-1-AP 1:1000) with HeLa cells lysate 2000ug.

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  IHC staining of human pancreas cancer using 24641-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 24641-1-AP (TOP2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 24641-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 24641-1-AP (TOP2A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 24641-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 24641-1-AP (TOP2A Antibody) at dilution of 1:50.

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  IHC staining of human breast cancer using 24641-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 24641-1-AP (TOP2A Antibody) at dilution of 1:50.

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  IF Staining of A431 using 24641-1-AP

  Immunofluorescent analysis of A431 cells using 24641-1-AP (TOP2A antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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  IF Staining of A431 using 24641-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using TOP2A antibody (24641-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HeLa-Zellen, HepG2-Zellen, MDA-MB-231-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

24641-1-AP bindet in WB, IP, IHC, IF, ELISA TOP2A und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TOP2A fusion protein Ag19551
• Vollständiger Name: topoisomerase (DNA) II alpha 170kDa
• Berechnetes Molekulargewicht: 1531 aa, 174 kDa
• Beobachtetes Molekulargewicht: 174 kDa
• GenBank-Zugangsnummer: BC140791
• Gene symbol: TOP2A
• Gene ID (NCBI): 7153
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TOP2A, also named as TOP2, belongs to the type II topoisomerase family. Topoisomerase II makes double-strand breaks. TOP2 control the topological states of DNA by transient breakage and subsequent rejoining of DNA strands. TOP2A may play a role in regulating the period length of ARNTL/BMAL1 transcriptional oscillation. DNA topoisomerase IIa (170 kDa, TOP2a/170) is essential in proliferating cells by resolving DNA topological entanglements during chromosome condensation, replication, and segregation.

Publikationen

Species	Application	Title
human	IHC	Front Cell Dev Biol; Chemotherapy- and Immune-Related Gene Panel in Prognosis Prediction and Immune Microenvironment of SCLC.; Authors - Meng-Yu Chen
human	WB,IF,IP	Mol Biol Cell; SPOP is essential for DNA-protein cross-link repair in prostate cancer cells: SPOP-dependent removal of topoisomerase 2A from the topoisomerase 2A-DNA cleavage complex.; Authors - Ryuta Watanabe
		Dev Biol; Identification of a key role for permeability glycoprotein in enhancing the cellular defense mechanisms of fertilized oocytes.; Authors - Jacinta H Martin
human	WB	Chem Biol Interact; SZC015, a synthetic oleanolic acid derivative, induces both apoptosis and autophagy in MCF-7 breast cancer cells.; Authors - Jingjun Wu
		Oncol Rep; Anticancer effects of β-elemene in gastric cancer cells and its potential underlying proteins: A proteomic study.; Authors - Jun-Song Liu
human	IHC	J Clin Neurosci; High prevalence of developmental venous anomaly in adult patients with midline thalamic diffuse gliomas.; Authors - Xingwang Zhou