TOM70 Monoklonaler Antikörper

TOM70 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

CloneNo.

1H8A1

Rezensionen (1)

Kat-Nr. : 66593-1-Ig

Synonyme

KIAA0719, KIAA0719, TOM70, TOM70, TOMM70A

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Galerie der Validierungsdaten

• 

  WB analysis using 66593-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66593-1-Ig (TOM70 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

• 

  IHC staining of mouse brain using 66593-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66593-1-Ig (TOM70 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66593-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66593-1-Ig (TOM70 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 66593-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66593-1-Ig (TOM70 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 66593-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66593-1-Ig (TOM70 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 66593-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66593-1-Ig (TOM70 antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, 4T1-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, PC-12-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:150-1:600
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

66593-1-Ig bindet in WB, IHC, IF, ELISA TOM70 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TOM70 fusion protein Ag6027
• Vollständiger Name: translocase of outer mitochondrial membrane 70 homolog A (S. cerevisiae)
• Berechnetes Molekulargewicht: 67 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC052994
• Gene symbol: TOMM70A
• Gene ID (NCBI): 9868
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TOM70 is a mitochondrial import receptor responsible for the import of cytosol synthesized precursor proteins into the mitochondria. Tom70 is a member of the tetratricopeptide repeat (TPR) co-chaperone family and contains a conserved TPR clamp domain for interaction with Hsc70 and Hsp90. Recently overexpression of TOM70 has been linked to chemoresistance to drugs in breast cancer cell lines. It is also believed that Tom70 is significantly associated with breast cancer relapse and may predict the recurrence of ovarian cancer.

Publikationen

Species	Application	Title
human	WB	Science; Structural insight into the SAM-mediated assembly of the mitochondrial TOM core complex.; Authors - Qiang Wang
human	WB	Mol Cell; Dynamic Regulation of Mitochondrial Import by the Ubiquitin System.; Authors - Lilian Phu
mouse	WB	Physiol Rep; TOM complex-independent transport pathway of myoglobin into mitochondria in C2C12 myotubes; Authors - Rikuhide Koma
mouse	IF	Asian J Androl; Caseinolytic mitochondrial matrix peptidase X is essential for homologous chromosome synapsis and recombination during meiosis of male mouse germ cells; Authors - Hai-Wei Feng ; KO Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Daniela (Verified Customer) (10-04-2022)

Good specific antibody that shows only one band at 75KDa in HeLa cell lysate (15ug/lane).

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa

TOM70 Antibody Western Blot,Immunoprecipitation validation (1:1000 dilution) in HeLa (Cat no:66593-1-Ig)