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TOM20 Rekombinanter Antikörper

TOM20 Rekombinant Antikörper für FC (Intra), IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, Huhn, human, Maus, Ratte, zabrafish

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D22

Kat-Nr. : CL488-80501

Synonyme

KIAA0016, MAS20, MOM19, TOM20, TOMM20

Galerie der Validierungsdaten

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated TOM20 Recombinant an (CL488-80501)

    IF Staining of HepG2 using CL488-80501

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 TOM20 antibody (CL488-80501, Clone: 1D22 ) at dilution of 1:200, CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated TOM20 Recombinant an (CL488-80501)

    FC experiment of HepG2 using CL488-80501

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TOM20 (CL488-80501, Clone:1D22) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in IFHepG2-Zellen
Erfolgreiche Detektion in FCHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-80501 bindet in IF, FC (Intra) TOM20 und zeigt Reaktivität mit Hausschwein, Huhn, human, Maus, Ratten, zabrafish

Getestete Reaktivität Hausschwein, Huhn, human, Maus, Ratte, zabrafish
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen TOM20 fusion protein Ag2378
Vollständiger Name translocase of outer mitochondrial membrane 20 homolog (yeast)
Berechnetes Molekulargewicht 145 aa, 16 kDa
Beobachtetes Molekulargewicht 16 kDa
GenBank-ZugangsnummerBC000882
Gene symbol TOMM20
Gene ID (NCBI) 9804
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TOM20, also named as KIAA0016, belongs to the Tom20 family. It is a central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22, TOM20 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore. TOM20 is characterized as major docking receptors to mediate the recognition by different mechanisms.

Protokolle

Produktspezifische Protokolle
IF protocol for CL Plus 488 TOM20 antibody CL488-80501Protokoll herunterladen
FC protocol for CL Plus 488 TOM20 antibody CL488-80501Protokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen