TOM20 Monoklonaler Antikörper

TOM20 Monoklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

19

CloneNo.

1D6F5

Rezensionen (1)

Kat-Nr. : 66777-1-Ig

Synonyme

Cell and organelle markers, KIAA0016, MAS20, Mitochondria Marker, Mitochondrial Marker, Mitochondrion Marker, MOM19, TOM20, TOMM20

---

Galerie der Validierungsdaten

• 

  WB analysis using 66777-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66777-1-Ig (TOM20 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 66777-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66777-1-Ig (TOM20 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66777-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66777-1-Ig (TOM20 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66777-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66777-1-Ig (TOM20 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66777-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66777-1-Ig (TOM20 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 66777-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TOM20 antibody (66777-1-Ig, Clone: 1D6F5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of HepG2 using 66777-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TOM20 antibody (66777-1-Ig, Clone: 1D6F5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), (CL594-Phalloidin, red).

• 

  FC experiment of HeLa using 66777-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TOM20 (66777-1-Ig, Clone:1D6F5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, THP-1-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:400-1:1600
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 12 publications below

Produktinformation

66777-1-Ig bindet in WB, IHC, IF, FC, ELISA TOM20 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TOM20 fusion protein Ag2378
• Vollständiger Name: translocase of outer mitochondrial membrane 20 homolog (yeast)
• Berechnetes Molekulargewicht: 145 aa, 16 kDa
• Beobachtetes Molekulargewicht: 16 kDa
• GenBank-Zugangsnummer: BC000882
• Gene symbol: TOMM20
• Gene ID (NCBI): 9804
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TOM20, also named as KIAA0016, belongs to the Tom20 family. It is a central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22, TOM20 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore. TOM20 is characterized as major docking receptors to mediate the recognition by different mechanisms.

Publikationen

Species	Application	Title
human	WB	Science; Structural insight into the SAM-mediated assembly of the mitochondrial TOM core complex.; Authors - Qiang Wang
human	IF	J Pineal Res; Melatonin and verteporfin synergistically suppress the growth and stemness of head and neck squamous cell carcinoma through the regulation of mitochondrial dynamics; Authors - Ye Young Shin
human		Brain; Inhibiting mitochondrial fission rescues degeneration in hereditary spastic paraplegia neurons.; Authors - Zhenyu Chen
	WB	Redox Biol; Hypoxic acclimation improves cardiac redox homeostasis and protects heart against ischemia-reperfusion injury through upregulation of O-GlcNAcylation.; Authors - Wei Ou
human	IF	Br J Cancer; Loss of NDUFS1 promotes gastric cancer progression by activating the mitochondrial ROS-HIF1α-FBLN5 signaling pathway; Authors - Tao Chen
human	IF	Cell Death Discov; Recombinant measles virus vaccine rMV-Hu191 exerts an oncolytic effect on esophageal squamous cell carcinoma via caspase-3/GSDME-mediated pyroptosis; Authors - Ailing Wu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Boyan (Verified Customer) (02-22-2024)

good for imaging experiments

• Applications: Immunofluorescence