TOB1 Polyklonaler Antikörper

TOB1 Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 14915-1-AP

Synonyme

APRO6, PIG49, Protein Tob1, TOB, TOB1, Transducer of erbB 2 1, transducer of ERBB2, 1, TROB, TROB1

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 14915-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14915-1-AP (TOB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14915-1-AP

  IP Result of anti-TOB1 (IP:14915-1-AP, 4ug; Detection:14915-1-AP 1:1000) with HeLa cells lysate 1600ug.

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  IHC staining of human stomach cancer using 14915-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14915-1-AP (TOB1 antibody) at dilution of 1:100 (under 0x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 14915-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14915-1-AP (TOB1 antibody) at dilution of 1:100 (under 0x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 14915-1-AP

  Immunofluorescent analysis of HeLa cells using 14915-1-AP (TOB1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

14915-1-AP bindet in WB, IP, IHC, IF, ELISA TOB1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TOB1 fusion protein Ag6714
• Vollständiger Name: transducer of ERBB2, 1
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 38-45 kDa
• GenBank-Zugangsnummer: BC031406
• Gene symbol: TOB1
• Gene ID (NCBI): 10140
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TOB1, also known as APRO6 or TROB1, is a member of the transducer of erbB-2/B-cell translocation gene protein family. Members of this family are anti-proliferative factors that have the potential to regulate cell growth. The TOB1 protein has a large additional C-terminal region, which contains PAM2 motifs that mediate interactions with poly(A)-binding proteins. The predicted MW of this protein is 38 kDa. Catalog#14915-1-AP recognises 38 and 45 kDa band, and the additional 45 kDa band may be due to phosphorylation.

Publikationen

Species	Application	Title
human	WB	Oncotarget; Screening key microRNAs for castration-resistant prostate cancer based on miRNA/mRNA functional synergistic network.; Authors - Jin Zhu
mouse	IHC	RNA Biol; NEDD4 triggers FOXA1 ubiquitination and promotes colon cancer progression under microRNA-340-5p suppression and ATF1 upregulation.; Authors - Meng Yue
human	WB	RNA Biol; Preferential translation of p53 target genes.; Authors - Miharu Hisaoka
human	WB	Am J Transl Res; Phosphorylation of TOB1 at T172 and S320 is critical for gastric cancer proliferation and progression.; Authors - Dong Wang
human	WB,IHC	Cancer Med; TOB1 suppresses proliferation in K-Ras wild-type pancreatic cancer.; Authors - Yuru Bai
mouse,human	WB,IF,IP	Commun Biol; TOB1 attenuates IRF3-directed antiviral responses by recruiting HDAC8 to specifically suppress IFN-β expression; Authors - Zhongxia Yu ; KO Validated