TNP1 Polyklonaler Antikörper

TNP1 Polyklonal Antikörper für IF, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

17

Rezensionen (2)

Kat-Nr. : 17178-1-AP

Synonyme

STP 1, TNP1, TP 1, TP1

---

Galerie der Validierungsdaten

• 

  WB analysis of mouse testis using 17178-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17178-1-AP (TNP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 17178-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17178-1-AP (TNP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of mouse testis using 17178-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using 17178-1-AP (TNP1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe
• Erfolgreiche Detektion in IF: Maushodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IHC: See 3 publications below
• IF: See 7 publications below

Produktinformation

17178-1-AP bindet in WB, IHC, IF, ELISA TNP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TNP1 fusion protein Ag10890
• Vollständiger Name: transition protein 1 (during histone to protamine replacement)
• Berechnetes Molekulargewicht: 55 aa, 7 kDa
• Beobachtetes Molekulargewicht: 6-10 kDa
• GenBank-Zugangsnummer: BC029516
• Gene symbol: TNP1
• Gene ID (NCBI): 7141
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Transition nuclear proteins (TNP1 and TNP2) are the major nuclear proteins that replace somatic histones during spermatogenesis. TNPs are required for normal chromatin condensation and functional sperm development, spermatogenesis was found to be compromised in both Tnp1 and Tnp2 null mice. TNP1, or TP1, localized in nucleus, is a spermatid-specific product of the haploid genome which replaces histone and is itself replaced in the mature sperm by the protamines. Recently, TNP-1 was used as a germ cell marker in condensing spermatids.

Publikationen

Species	Application	Title
mouse	WB	Cell; Ubiquitination-Deficient Mutations in Human Piwi Cause Male Infertility by Impairing Histone-to-Protamine Exchange during Spermiogenesis.; Authors - Lan-Tao Gou
human	WB	Mol Cell; Chromodomain Protein CDYL Acts as a Crotonyl-CoA Hydratase to Regulate Histone Crotonylation and Spermatogenesis.; Authors - Shumeng Liu
mouse	IF,IHC	J Hazard Mater; Multi-omics analyses reveal the mechanisms of Arsenic-induced male reproductive toxicity in mice.; Authors - Zijun Peng
mouse	IF	Elife; Absence of CEP78 causes photoreceptor and sperm flagella impairments in mice and a human individual; Authors - Tianyu Zhu
mouse	WB	Environ Pollut; Silica nanoparticles induce spermatogenesis disorders via L3MBTL2-DNA damage-p53 apoptosis and RNF8-ubH2A/ubH2B pathway in mice.; Authors - Jianhui Liu
mouse	WB	Environ Pollut; Silica nanoparticles inhibiting the differentiation of round spermatid and chromatin remodeling of haploid period via MIWI in mice.; Authors - Jianhui Liu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Chongil (Verified Customer) (08-19-2021)

This is a great IF antibody to detect Tnp1 protein.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:800
• Cell Tissue Type: Mouse testis section

TNP1 Antibody Immunofluorescence validation (1:800 dilution) in Mouse testis section (Cat no:17178-1-AP)

FH

Zhenminm (Verified Customer) (07-08-2019)

This antibody is highly specific and works excellently for immunohistochemistry as well as immunofluorescence in formalin fixed and paraffin embedded tissue sections.

• Applications: Immunohistochemistry, Immunofluorescence,
• Primary Antibody Dilution: 1:1,000
• Cell Tissue Type: Mouse Testis

TNP1 Antibody Immunohistochemistry,Immunofluorescence, validation (1:1,000 dilution) in Mouse Testis (Cat no:17178-1-AP)