TNNI1 Polyklonaler Antikörper

TNNI1 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Rezensionen (1)

Kat-Nr. : 16102-1-AP

Synonyme

SSTNI, TNN1, TNNI1

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Galerie der Validierungsdaten

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  WB analysis of human skeletal muscle using 16102-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16102-1-AP (TNNI1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16102-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16102-1-AP (TNNI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skeletal muscle using 16102-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16102-1-AP (TNNI1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 16102-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16102-1-AP (TNNI1 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe, humanes Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IF: See 2 publications below

Produktinformation

16102-1-AP bindet in WB, IHC, IF, ELISA TNNI1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TNNI1 fusion protein Ag9001
• Vollständiger Name: troponin I type 1 (skeletal, slow)
• Berechnetes Molekulargewicht: 187 aa, 22 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC012600
• Gene symbol: TNNI1
• Gene ID (NCBI): 7135
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
rat	WB,IF	Basic Res Cardiol; Alkaline nucleoplasm facilitates contractile gene expression in the mammalian heart.; Authors - Alzbeta Hulikova
human	WB	iScience; Engineering of thick human functional myocardium via static stretching and electrical stimulation.; Authors - Danielle Pretorius
mouse	WB	Sci Rep; A missense mutation in the RSRSP stretch of Rbm20 causes dilated cardiomyopathy and atrial fibrillation in mice.; Authors - Kensuke Ihara
mouse	WB	Front Physiol; Lipidomic Analyses Reveal Specific Alterations of Phosphatidylcholine in Dystrophic Mdx Muscle.; Authors - William J Valentine
human	WB	Cell Death Discov; Long noncoding RNA LINC00284 facilitates cell proliferation in papillary thyroid cancer via impairing miR-3127-5p targeted E2F7 suppression.; Authors - Bin Zhou
mouse	IF	Front Med; Distinct mononuclear diploid cardiac subpopulation with minimal cell-cell communications persists in embryonic and adult mammalian heart; Authors - Miaomiao Zhu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Xuebin (Verified Customer) (11-12-2018)

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: heart