TNIP2 Polyklonaler Antikörper

TNIP2 Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 15459-1-AP

Synonyme

ABIN 2, ABIN2, FLIP1, KLIP, TNFAIP3 interacting protein 2, TNIP2

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 15459-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15459-1-AP (TNIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 15459-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15459-1-AP (TNIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 15459-1-AP

  IP Result of anti-TNIP2 (IP:15459-1-AP, 4ug; Detection:15459-1-AP 1:500) with HepG2 cells lysate 3000ug.

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  IHC staining of human liver using 15459-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15459-1-AP (TNIP2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 15459-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15459-1-AP (TNIP2 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 15459-1-AP

  Immunofluorescent analysis of HepG2 cells, using TNIP2 antibody 15459-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Mauslebergewebe
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IF: See 2 publications below

Produktinformation

15459-1-AP bindet in WB, IP, IHC, IF, ELISA TNIP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TNIP2 fusion protein Ag7731
• Vollständiger Name: TNFAIP3 interacting protein 2
• Berechnetes Molekulargewicht: 49 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC002740
• Gene symbol: TNIP2
• Gene ID (NCBI): 79155
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TNIP2, also named as ABIN2 and FLIP1, inhibits NF-kappa-B activation by blocking the interaction of RIPK1 with its downstream effector NEMO/IKBKG. It acts as transcriptional coactivator when translocated to the nucleus. TNIP2 has some isoforms with MW 49 kDa and 37 kDa.

Publikationen

Species	Application	Title
mouse	WB	Sci Signal; The kinase TPL2 activates ERK and p38 signaling to promote neutrophilic inflammation.; Authors - Kate Senger
pig,mouse	WB	J Virol; Foot-and-Mouth Disease Virus Structural Protein VP1 Destroys the Stability of TPL2 Trimer by Degradation TPL2 to Evade Host Antiviral Immunity.; Authors - Keshan Zhang
human,mouse	WB,IF	Neurosci Lett; TNIP2 inhibits amyloidogenesis by regulating the 3'UTR of BACE1: an in vitro study; Authors - Long Chen
rat	IF	Neuropeptides; Protective effect of TNIP2 on the inflammatory response of microglia after spinal cord injury in rats; Authors - Jiawei Fu
human	WB	Immun Inflamm Dis; TNFAIP3 interacting protein 2 relieves lipopolysaccharide (LPS)-induced inflammatory injury in endometritis by inhibiting NF-kappaB activation; Authors - Xinxin Qian