TNIP1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TNIP1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(16)

Rezensionen (1)

Kat-Nr. : 15104-1-AP

Synonyme

ABIN1, A20-binding inhibitor of NF-kappa-B activation 1, ABIN 1, ABIN-1, HIV-1 Nef-interacting protein

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• 

  WB analysis using 15104-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15104-1-AP (TNIP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 15104-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15104-1-AP (TNIP1 Antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 15104-1-AP

  IP result of anti-TNIP1 (IP:15104-1-AP, 4ug; Detection:15104-1-AP 1:6000) with HeLa cells lysate 1320 ug.

• 

  IHC staining of human lymphoma using 15104-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 15104-1-AP (TNIP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 15104-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 15104-1-AP (TNIP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 15104-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TNIP1 antibody (15104-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HEK-293-Zellen, HeLa-Zellen, Maus-Skelettmuskelgewebe, U2OS-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 13 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Produktinformation

15104-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA TNIP1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TNIP1 fusion protein Ag1561
• Vollständiger Name: TNFAIP3 interacting protein 1
• Berechnetes Molekulargewicht: 72 kDa
• Beobachtetes Molekulargewicht: 72 kDa
• GenBank-Zugangsnummer: BC014008
• Gene symbol: TNIP1
• Gene ID (NCBI): 10318
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TNIP1, also named as KIAA0113, NAF1, Nip40-1, VAN and ABIN-1, is involved in inhibition of nuclear factor- B (NF- B) activation by interacting with TNF alpha-induced protein 3, A20 (TNFAIP3). TNIP1 repression of RARs in the presence of RA places it in a small category of corepressors of agonist-bound NRs.

Publikationen

Species	Application	Title
human	IF	Mol Cell; TNIP1 inhibits selective autophagy via bipartite interaction with LC3/GABARAP and TAX1BP1; Authors - François Le Guerroué
mouse	WB	Cell Death Differ; Ubiquitin-binding domain in ABIN1 is critical for regulating cell death and inflammation during development.; Authors - Ming Li
human	WB	BMC Biol; A functional genomics screen for microRNA regulators of NF-kappaB signaling.; Authors - Olarerin-George Anthony O AO ; KD Validated
human	WB,IF	Retrovirology; Immune regulator ABIN1 suppresses HIV-1 transcription by negatively regulating the ubiquitination of Tat.; Authors - Shiyou Chen ; KD Validated
human	WB	J Cell Biochem; ABIN1 inhibits HDAC1 ubiquitination and protects HDAC1 from both proteasome- and lysozyme-dependent degradation.; Authors - Yuhong Ma ; KO Validated
mouse	WB	Clin Exp Pharmacol Physiol; ABIN-1 protects chondrocytes from lipopolysaccharide-induced inflammatory injury through the inactivation of NF-κB signalling.; Authors - Kan Peng

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Heiko (Verified Customer) (09-04-2019)

PVDF membrane blocked in 5% milk TBST 1 h at RT.Primary antibody incubation overnight 4°C.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa cells

TNIP1 Antibody Western Blot, validation (1:1000 dilution) in HeLa cells (Cat no:15104-1-AP)