TIPE2 Polyklonaler Antikörper

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• KD/KO Validated

TIPE2 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

35

Kat-Nr. : 15940-1-AP

Synonyme

Inflammation factor protein 20, TIPE2, TNFAIP8 like protein 2, TNFAIP8L2

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Galerie der Validierungsdaten

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  WB analysis using 15940-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15940-1-AP (TIPE2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 15940-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 15940-1-AP (TIPE2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat lymph using 15940-1-AP

  rat lymph tissue were subjected to SDS PAGE followed by western blot with 15940-1-AP (TIPE2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 15940-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 15940-1-AP (TIPE2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse small intestine using 15940-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 15940-1-AP (TIPE2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse cerebellum using 15940-1-AP

  IP Result of anti-TIPE2 (IP:15940-1-AP, 3ug; Detection:15940-1-AP 1:500) with mouse cerebellum tissue lysate 4000ug.

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  IHC staining of human lymphoma using 15940-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 15940-1-AP (TIPE2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 15940-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 15940-1-AP (TIPE2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 15940-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 15940-1-AP (TIPE2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 15940-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 15940-1-AP (TIPE2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 15940-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor slide using 15940-1-AP (TIPE2 antibody at dilution of 1:50.

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  IHC staining of human ovary tumor using 15940-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor slide using 15940-1-AP (TIPE2 antibody at dilution of 1:50.

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  IF Staining of RAW 264.7 using 15940-1-AP

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using TIPE2 antibody (15940-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 15940-1-AP

  Immunofluorescent analysis of HepG2 cells using 15940-1-AP (TIPE2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of THP-1 using 15940-1-AP

  1X10^6 THP-1 cells were intracellularly stained with 0.4 ug Anti-Human TIPE2 (15940-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: THP-1-Zellen, Maus-Cerebellum-Gewebe, Maus-Dünndarmgewebe, Mausmilzgewebe, Rattenlymphgewebe, Rattenmilzgewebe, RAW 264.7-Zellen
• Erfolgreiche IP: Maus-Cerebellum-Gewebe
• Erfolgreiche Detektion in IHC: humanes Lymphomgewebe, humanes Ovarialkarzinomgewebe, Mausmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: RAW 264.7-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in FC: THP-1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 10 publications below
• WB: See 31 publications below
• IHC: See 12 publications below
• IF: See 10 publications below
• IP: See 2 publications below

Produktinformation

15940-1-AP bindet in WB, IP, IHC, IF, FC, ELISA TIPE2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TIPE2 fusion protein Ag7964
• Vollständiger Name: tumor necrosis factor, alpha-induced protein 8-like 2
• Berechnetes Molekulargewicht: 184 aa, 21 kDa
• Beobachtetes Molekulargewicht: 18-20 kDa
• GenBank-Zugangsnummer: BC063014
• Gene symbol: TNFAIP8L2
• Gene ID (NCBI): 79626
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TIPE2 (TNF-α-inducible protein 8-like 2, or TNFAIP8L2), a negative immune regulator, is essential for maintaining immune homeostasis. TIPE2 shares considerable sequence homology with members of the tumor necrosis factor-α-inducible protein 8 (TNFAIP8) family, which are thought to regulate cellular and immune homeostasis. TIPE2 acts as a negative regulator of Toll-like receptor and T-cell receptor function and is preferentially expressed in lymphoid tissues. TIPE2 plays an essential role for a signal transduction pathway that links inflammatory immune response to specific conditions after cerebral ischemia

Publikationen

Species	Application	Title
human	WB	Nat Immunol; Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity.; Authors - Li Jianhua J
mouse	WB,IF,IP	Nat Immunol; Direction of leukocyte polarization and migration by the phosphoinositide-transfer protein TIPE2.; Authors - Svetlana A Fayngerts ; KO Validated
mouse,human	WB	Sci Adv; TIPE2 is a checkpoint of natural killer cell maturation and antitumor immunity.; Authors - Jiacheng Bi ; KO Validated
mouse	WB	Autophagy; TNFAIP8L2/TIPE2 impairs autolysosome reformation via modulating the RAC1-MTORC1 axis.; Authors - Wen Li ; KO Validated
mouse	WB	J Exp Med; TIPE2 specifies the functional polarization of myeloid-derived suppressor cells during tumorigenesis.; Authors - Dehong Yan ; KO Validated
human,mouse	WB	Cancer Immunol Res; Tumor Necrosis Factor-α-Induced Protein 8-Like 2 Fosters Tumor-Associated Microbiota to Promote the Development of Colorectal Cancer.; Authors - Yunwei Lou