TNFAIP3 Monoklonaler Antikörper

TNFAIP3 Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

3A11G6

Kat-Nr. : CL594-66695

Synonyme

A20, OTUD7C, TNF alpha induced protein 3, TNFA1P2, TNFAIP3, Zinc finger protein A20

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Galerie der Validierungsdaten

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  IF Staining of HeLa using CL594-66695

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594-conjugated TNFAIP3 antibody (CL594-66695, Clone: 3A11G6) at dilution of 1:200, F-actin was stained using CL488-phalloidin (green) and DNA was stained by DAPI (blue).

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  FC experiment of HeLa using CL594-66695

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human TNFAIP3 (CL594-66695, Clone:3A11G6) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-66695 bindet in IF, FC (Intra) TNFAIP3 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TNFAIP3 fusion protein Ag18150
• Vollständiger Name: tumor necrosis factor, alpha-induced protein 3
• Berechnetes Molekulargewicht: 790 aa, 90 kDa
• Beobachtetes Molekulargewicht: 80 kDa
• GenBank-Zugangsnummer: BC114480
• Gene symbol: TNFAIP3
• Gene ID (NCBI): 7128
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TNFAIP3, also named A20, is a cytoplasmic zinc finger protein that inhibits nuclear factor kappa-B (NFKB) activity and tumor necrosis factor (TNF)-mediated programmed cell death. A20 is a ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities, and is involved in immune and inflammatory responses signaled by cytokines, such as TNF-alpha and IL-1 beta, or pathogens via Toll-like receptors (TLRs) through terminating NF-kappa-B activity.

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C