TNFAIP3 Monoklonaler Antikörper

TNFAIP3 Monoklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

CloneNo.

3A11G6

Kat-Nr. : 66695-1-Ig

Synonyme

A20, OTUD7C, TNF alpha induced protein 3, TNFA1P2, TNFAIP3, Zinc finger protein A20

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Galerie der Validierungsdaten

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  WB analysis using 66695-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66695-1-Ig (TNFAIP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 66695-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66695-1-Ig (TNFAIP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse kidney using 66695-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66695-1-Ig (TNFAIP3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 66695-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66695-1-Ig (TNFAIP3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 66695-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66695-1-Ig (TNFAIP3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 66695-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66695-1-Ig (TNFAIP3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat ovary using 66695-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 66695-1-Ig (TNFAIP3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat ovary using 66695-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 66695-1-Ig (TNFAIP3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 66695-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66695-1-Ig (TNFAIP3 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HeLa using 66695-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human TNFAIP3 (66695-1-Ig, Clone:3A11G6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: Mausnierengewebe, humanes Schilddrüsenkarzinomgewebe, Ratten-Eierstockgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Produktinformation

66695-1-Ig bindet in WB, IHC, IF, FC, ELISA TNFAIP3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TNFAIP3 fusion protein Ag18150
• Vollständiger Name: tumor necrosis factor, alpha-induced protein 3
• Berechnetes Molekulargewicht: 790 aa, 90 kDa
• Beobachtetes Molekulargewicht: 80 kDa
• GenBank-Zugangsnummer: BC114480
• Gene symbol: TNFAIP3
• Gene ID (NCBI): 7128
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TNFAIP3, also named A20, is a cytoplasmic zinc finger protein that inhibits nuclear factor kappa-B (NFKB) activity and tumor necrosis factor (TNF)-mediated programmed cell death. A20 is a ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities, and involved in immune and inflammatory responses signaled by cytokines, such as TNF-alpha and IL-1 beta, or pathogens via Toll-like receptors (TLRs) through terminating NF-kappa-B activity.

Publikationen

Species	Application	Title
mouse	IHC	Biology (Basel); Interleukin-10 Deficiency Impacts on TNF-Induced NFκB Regulated Responses In Vivo; Authors - Stamatia Papoutsopoulou
human	WB,IHC,IF	Cell Death Dis; A20 targets PFKL and glycolysis to inhibit the progression of hepatocellular carcinoma.; Authors - Yilu Feng
human,mouse	IHC,IF	Sci Total Environ; Alpha-ketoglutarate alleviates cadmium-induced inflammation by inhibiting the HIF1A-TNFAIP3 pathway in hepatocytes; Authors - Yinzhao Jia