TNFAIP3 Polyklonaler Antikörper
TNFAIP3 Polyklonal Antikörper für WB,ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte und mehr (1)
Anwendung
WB, IHC, IF, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 23456-1-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | Jurkat-Zellen, HeLa-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
WB | See 16 publications below |
IHC | See 1 publications below |
IF | See 2 publications below |
Produktinformation
23456-1-AP bindet in WB, IHC, IF, ELISA TNFAIP3 und zeigt Reaktivität mit human, Maus, Ratten
Getestete Reaktivität | human, Maus, Ratte |
In Publikationen genannte Reaktivität | human, Hausschwein, Maus, Ratte |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | TNFAIP3 fusion protein Ag18150 |
Vollständiger Name | tumor necrosis factor, alpha-induced protein 3 |
Berechnetes Molekulargewicht | 790 aa, 90 kDa |
Beobachtetes Molekulargewicht | 80-90 kDa, 37-40 kDa |
GenBank-Zugangsnummer | BC114480 |
Gene symbol | TNFAIP3 |
Gene ID (NCBI) | 7128 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
TNFAIP3, also named A20, is a cytoplasmic zinc finger protein that inhibits nuclear factor kappa-B (NFKB) activity and tumor necrosis factor (TNF)-mediated programmed cell death. A20 is a ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities, and involved in immune and inflammatory responses signaled by cytokines, such as TNF-alpha and IL-1 beta, or pathogens via Toll-like receptors (TLRs) through terminating NF-kappa-B activity. Upon T- or B-cell receptor engagement human (h) A20 is cleaved by MALT1 after arginine 439, yielding an N-terminal fragment and a C-terminal one (~37-40 kDa). This antibody, which is a rabbit polyclonal antibody raised against 350 aa of C-terminus of human TNFAIP3, can recognize full-length A20 (~90 kDa) and cleaved form (~37-40 kDa).
Protokolle
Produktspezifische Protokolle | |
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WB protocol for TNFAIP3 antibody 23456-1-AP | Protokoll herunterladen |
Standard-Protokolle | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Sci Total Environ USP15 participates in DBP-induced testicular oxidative stress injury through regulating the Keap1/Nrf2 signaling pathway. | ||
Am J Cancer Res ZY-444 inhibits the growth and metastasis of prostate cancer by targeting TNFAIP3 through TNF signaling pathway | ||
Biomedicines The Cholinergic Anti-Inflammatory Pathway Attenuates the Development of Atherosclerosis in Apoe-/- Mice through Modulating Macrophage Functions. | ||
Biomedicines Sertoli Cells Express Accommodation, Survival, and Immunoregulatory Factors When Exposed to Normal Human Serum | ||
Int Immunopharmacol TNFAIP3-upregulated RIP3 exacerbates acute pancreatitis via activating NLRP3 inflammasome. | ||
Mol Cell Biol LncRNA HOXA-AS3 integrates NF-κB signaling to regulate endothelium inflammation. |