TNFAIP1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TNFAIP1 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 15320-1-AP

Synonyme

B12, B61, BACURD2, EDP1, hBACURD2, Protein B12, TNFAIP1

---

Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 15320-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15320-1-AP (TNFAIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 15320-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 15320-1-AP (TNFAIP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 15320-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15320-1-AP (TNFAIP1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 15320-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15320-1-AP (TNFAIP1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 15320-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15320-1-AP (TNFAIP1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 15320-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15320-1-AP (TNFAIP1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 15320-1-AP

  Immunofluorescent analysis of HeLa cells using 15320-1-AP (TNFAIP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, L02-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:300-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 3 publications below

Produktinformation

15320-1-AP bindet in WB, IHC, IF, ELISA TNFAIP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TNFAIP1 fusion protein Ag0220
• Vollständiger Name: tumor necrosis factor, alpha-induced protein 1 (endothelial)
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC001949
• Gene symbol: TNFAIP1
• Gene ID (NCBI): 7126
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TNFAIP1 was identified as a human gene activated transcriptionally by tumour necrosis factor alpha in endothelial cells. It is encoded by a 3.5-kilobase transcript and its expression is found to be developmentally regulated in a tissue-specific manner. The open reading frame predicts a 316-amino acid sequence rich in charged residues, particularly at the carboxyl terminus.

Publikationen

Species	Application	Title
mouse	WB	Neurotox Res; TNFAIP1 Mediates Formaldehyde-Induced Neurotoxicity by Inhibiting the Akt/CREB Pathway in N2a Cells.; Authors - Junzhi Yi ; KD Validated
human	WB	Oncol Lett; MicroRNA-373 is upregulated and targets TNFAIP1 in human gastric cancer, contributing to tumorigenesis.; Authors - Zhang Xiaoting X ; KD Validated
	WB	Cell Commun Signal; Neuronal-specific TNFAIP1 ablation attenuates postoperative cognitive dysfunction via targeting SNAP25 for K48-linked ubiquitination; Authors - Wei Wang