TNF Alpha Monoklonaler Antikörper

TNF Alpha Monoklonal Antikörper für IF

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IF

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Publikationen

2

CloneNo.

7B8A11

Kat-Nr. : CL488-60291

Synonyme

Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNFSF2, Tumor necrosis factor

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Galerie der Validierungsdaten

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  IF Staining of human breast cancer using CL488-60291

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CL488-60291 (TNF alpha antibody) at dilution of 1:50.

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  IF Staining of human breast cancer using CL488-60291

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CL488-60291 (TNF alpha antibody) at dilution of 1:50.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: humanes Mammakarzinomgewebe

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

CL488-60291 bindet in WB, IF TNF Alpha und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TNF Alpha fusion protein Ag11413
• Vollständiger Name: tumor necrosis factor (TNF superfamily, member 2)
• Berechnetes Molekulargewicht: 233 aa, 26 kDa
• GenBank-Zugangsnummer: BC028148
• Gene symbol: TNF
• Gene ID (NCBI): 7124
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, INS resistance, and cancer. The antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

Publikationen

Species	Application	Title
	WB	Biomed Pharmacother; Nr2e1 ablation impairs liver glucolipid metabolism and induces inflammation, high-fat diets amplify the damage.; Authors - Qing Xiong
mouse	IF	Biomacromolecules; Multifunctional Polymer Vesicles for Synergistic Antibiotic-Antioxidant Treatment of Bacterial Keratitis; Authors - Qiumeng Chen