TNC/Tenascin-C Monoklonaler Antikörper

TNC/Tenascin-C Monoklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

CloneNo.

1F7G8

Rezensionen (1)

Kat-Nr. : 67710-1-Ig

Synonyme

Cytotactin, GMEM, GP 150 225, Hexabrachion, HXB, JI, Myotendinous antigen, Neuronectin, Tenascin, tenascin C, TN, TN C, TNC

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Galerie der Validierungsdaten

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  WB analysis using 67710-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67710-1-Ig (TNC/Tenascin-C antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat cerebellum using 67710-1-Ig

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 67710-1-Ig (TNC/Tenascin-C antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 67710-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67710-1-Ig (TNC/Tenascin-C antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 67710-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67710-1-Ig (TNC/Tenascin-C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 67710-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67710-1-Ig (TNC/Tenascin-C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67710-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67710-1-Ig (TNC/Tenascin-C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67710-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67710-1-Ig (TNC/Tenascin-C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 67710-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67710-1-Ig (TNC/Tenascin-C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 67710-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67710-1-Ig (TNC/Tenascin-C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67710-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67710-1-Ig (TNC/Tenascin-C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67710-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67710-1-Ig (TNC/Tenascin-C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 67710-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67710-1-Ig (TNC/Tenascin-C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 67710-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67710-1-Ig (TNC/Tenascin-C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenhirngewebe, HEK-293-Zellen, Maushirngewebe, Ratten-Cerebellum-Gewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Leberkarzinomgewebe, humanes Lungenkarzinomgewebe, Maushirngewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

67710-1-Ig bindet in WB, IHC, IF, ELISA TNC/Tenascin-C und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TNC/Tenascin-C fusion protein Ag21412
• Vollständiger Name: tenascin C
• Berechnetes Molekulargewicht: 2201 aa, 241 kDa
• Beobachtetes Molekulargewicht: 260 kDa
• GenBank-Zugangsnummer: BC151843
• Gene symbol: TNC
• Gene ID (NCBI): 3371
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tenascin-C (TNC) is a large hexameric extracellular matrix glycoprotein of the tenascin family (PMID: 21818551). It is a multimodular protein containing multiple epidermal growth factor (EGF)-like repeats and fibronectin type III (FN III) domains (PMID: 1719530). Tenascin-C is highly expressed during embryonic development, particularly in the developing central nervous system, around motile cells and at epithelial-mesenchymal interaction sites (PMID: 25738825). In adult tissues, the expression and the distribution of TNC are typically limited under normal physiological conditions. It is upregulated during injury, inflammation, regeneration, and cancer (PMID: 7694605; 25120494). Tenascin-C is a diverse protein that can produce different functions including regulating cell adhesion, migration and proliferation.

Publikationen

Species	Application	Title
rat	WB,IHC	Oxid Med Cell Longev; Eugenol-Preconditioned Mesenchymal Stem Cell-Derived Extracellular Vesicles Promote Antioxidant Capacity of Tendon Stem Cells In Vitro and In Vivo.; Authors - Xiangze Li
rat	WB,IF	Acta Biomater; Rejuvenation of tendon stem/progenitor cells for functional tendon regeneration through platelet-derived exosomes loaded with recombinant Yap1; Authors - Jinwei Lu
mouse	IHC	Front Endocrinol (Lausanne); Identification of diagnostic markers related to oxidative stress and inflammatory response in diabetic kidney disease by machine learning algorithms: Evidence from human transcriptomic data and mouse experiments; Authors - Ming Zhong
human	WB	Cell Death Dis; CEBPD is a master transcriptional factor for hypoxia regulated proteins in glioblastoma and augments hypoxia induced invasion through extracellular matrix-integrin mediated EGFR/PI3K pathway; Authors - Xing-Gang Mao
mouse	WB	Mol Biotechnol; NT-3 Combined with TGF-β Signaling Pathway Enhance the Repair of Spinal Cord Injury by Inhibiting Glial Scar Formation and Promoting Axonal Regeneration; Authors - Taibang Chen
rat	IF	J Nanobiotechnology; 3D-printed hydrogel particles containing PRP laden with TDSCs promote tendon repair in a rat model of tendinopathy; Authors - Congsun Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Balawant (Verified Customer) (12-02-2022)

This antibody is great for western blot as well as for Immunofluorescence.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000 (WB) and 1:500 (IF)
• Cell Tissue Type: Colon Tissue of mice and caco2 cell