TMEM98 Polyklonaler Antikörper

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• KD/KO Validated

TMEM98 Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 14731-1-AP

Synonyme

Protein TADA1, TMEM98, transmembrane protein 98

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Galerie der Validierungsdaten

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  WB analysis of mouse E12.5 embryos using 14731-1-AP

  WB result of anti-TMEM98(14731-1-AP) in WT and KO mouse (10 ug of total protein lysate from E12.5 embryos) by Dr. Sally H. Cross.

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  IP experiment of Y79 using 14731-1-AP

  IP result of anti-TMEM98 (IP:14731-1-AP, 4ug; Detection:14731-1-AP 1:300) with Y79 cells lysate 2400 ug.

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  IHC staining of mouse embryo using 14731-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 14731-1-AP (TMEM98 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-E12.5-Embryos
• Erfolgreiche IP: Y79-Zellen
• Erfolgreiche Detektion in IHC: Mausembryogewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Produktinformation

14731-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA TMEM98 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TMEM98 fusion protein Ag6492
• Vollständiger Name: transmembrane protein 98
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 21-25 kDa
• GenBank-Zugangsnummer: BC000526
• Gene symbol: TMEM98
• Gene ID (NCBI): 26022
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TMEM98, also named as TADA1, composed of 226 amino acid residues, is a potential single-pass transmembrane protein. It is expression in both nucleus and cytoplasm. TMEM98 was found to be over-expressed in most of the hepatocellular carcinoma tissue. MEM98 is not only a prognostic marker for patients with hepatocellular carcinoma but also a novel molecular target associated with intrinsic and acquired chemoresistance of hepatocellular carcinoma (PMID: 24608572).

Publikationen

Species	Application	Title
mouse	WB,IF	PLoS Genet; The nanophthalmos protein TMEM98 inhibits MYRF self-cleavage and is required for eye size specification.; Authors - Sally H Cross
human	IF	PLoS Genet; Variants in myelin regulatory factor (MYRF) cause autosomal dominant and syndromic nanophthalmos in humans and retinal degeneration in mice.; Authors - Sarah J Garnai
human	WB	Aging (Albany NY); The microRNA miR-29c-5p inhibits cell proliferation and migration by targeting TMEM98 in head and neck carcinoma.; Authors - Jingjia Li
mouse	WB,IF	Invest Ophthalmol Vis Sci; Missense Mutations in the Human Nanophthalmos Gene TMEM98 Cause Retinal Defects in the Mouse.; Authors - Sally H Cross ; KO Validated
human	WB,CoIP	PLoS One; TMEM98 is a negative regulator of FRAT mediated Wnt/ß-catenin signalling.; Authors - Tanne van der Wal
human	WB,IHC	Front Biosci (Landmark Ed); Tumor-Suppressor Gene Transmembrane Protein 98 Promotes Proliferation and Inhibits Apoptosis in Ovarian Cancer; Authors - San-Gang Wu