TMEM74 Monoklonaler Antikörper

TMEM74 Monoklonal Antikörper für WB,ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB,ELISA

Konjugation

Unkonjugiert

CloneNo.

2H1B7

Kat-Nr. : 67773-1-Ig

Synonyme

FLJ30668, TMEM74, transmembrane protein 74

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Galerie der Validierungsdaten

• 

  WB analysis of LNCaP using 67773-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67773-1-Ig (TMEM74 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 67773-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 67773-1-Ig (TMEM74 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 67773-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67773-1-Ig (TMEM74 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, HEK-293-Zellen, L02-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67773-1-Ig bindet in WB,ELISA TMEM74 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TMEM74 fusion protein Ag22359
• Vollständiger Name: transmembrane protein 74
• Berechnetes Molekulargewicht: 305 aa, 33 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC030710
• Gene symbol: TMEM74
• Gene ID (NCBI): 157753
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Transmembrane protein 74 (TMEM74), plays an essential role in autophagy during cell starvation and other stress conditions. TMEM74 is localized to the lysosome and autophagosome. It has been rreported that TMEM74-induced autophagy may be associated with PI3K signal transduction. (PMID: 19029833, 18294959)