TMEM70 Polyklonaler Antikörper

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• KD/KO Validated

TMEM70 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 20388-1-AP

Synonyme

TMEM70, transmembrane protein 70

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Galerie der Validierungsdaten

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  WB analysis of A2780 using 20388-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 20388-1-AP (TMEM70 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 20388-1-AP

  WB result of TMEM70 antibody (20388-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM70 transfected Jurkat cells.

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  WB analysis of A2780 using 20388-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 20388-1-AP (TMEM70 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 20388-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20388-1-AP (TMEM70 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 20388-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20388-1-AP (TMEM70 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A2780-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below

Produktinformation

20388-1-AP bindet in WB, IHC, ELISA TMEM70 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TMEM70 fusion protein Ag14226
• Vollständiger Name: transmembrane protein 70
• Berechnetes Molekulargewicht: 260 aa, 29 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: BC002748
• Gene symbol: TMEM70
• Gene ID (NCBI): 54968
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TMEM70 belongs to the TMEM70 family. It is involved in biogenesis of mitochondrial ATP synthase. Defects in TMEM70 are a cause of mitochondrial encephalocardiomyopathy neonatal due to ATP synthase deficiency (MT-ATPSD). A publication(PMID:21147908) identifies that TMEM70 gene defect as a pan-ethnic disorder and further redefines it as the most common cause of nuclear-origin ATP synthase deficiency.

Publikationen

Species	Application	Title
human	WB	Mitochondrion; Mitochondrial membrane assembly of TMEM70 protein.; Authors - Hana Kratochvílová
human	WB	Neurogenetics; TMEM70: a mutational hot spot in nuclear ATP synthase deficiency with a pivotal role in complex V biogenesis.; Authors - Torraco Alessandra A
human	WB	JIMD Rep; Common and Novel TMEM70 Mutations in a Cohort of Italian Patients with Mitochondrial Encephalocardiomyopathy.; Authors - Daria Diodato
human	WB	Life Sci Alliance; Mitochondrial stress response triggered by defects in protein synthesis quality control.; Authors - Uwe Richter ; KD Validated