TMEM192 Polyklonaler Antikörper

TMEM192 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Rezensionen (1)

Kat-Nr. : 28263-1-AP

Synonyme

FLJ38482, TMEM192, transmembrane protein 192

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Galerie der Validierungsdaten

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  WB analysis using 28263-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28263-1-AP (TMEM192 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 28263-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28263-1-AP (TMEM192 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 28263-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28263-1-AP (TMEM192 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 28263-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28263-1-AP (TMEM192 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 28263-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28263-1-AP (TMEM192 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 28263-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28263-1-AP (TMEM192 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 28263-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28263-1-AP (TMEM192 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 28263-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28263-1-AP (TMEM192 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 28263-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28263-1-AP (TMEM192 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 28263-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28263-1-AP (TMEM192 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 28263-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28263-1-AP (TMEM192 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Leberkarzinomgewebe, humanes Lungenkarzinomgewebe, humanes Prostatakarzinomgewebe, Maus-Cerebellum-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

28263-1-AP bindet in WB, IHC, ELISA TMEM192 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TMEM192 fusion protein Ag27576
• Vollständiger Name: transmembrane protein 192
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC036301
• Gene symbol: TMEM192
• Gene ID (NCBI): 201931
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Transmembrane protein 192 (TMEM192) has been previously identified in proteomic analyses of lysosomal membranes.TMEM192 shows a wide expression pattern in mouse tissues. Interestingly, TMEM192 was found to be highly expressed in tumor cell lines, while it was not expressed or detected at low levels in normal cell lines(PMID: 22736246). TMEM192 can be detected as 30 kDa.

Publikationen

Species	Application	Title
human	IHC	Mol Ther; Polyphyllin D "Punctures" Hypertrophic Lysosomes to Reverse Drug Resistance of Hepatocellular Carcinoma by Targeting Acid Sphingomyelinase; Authors - Yang Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

H (Verified Customer) (09-27-2020)

This antibody is very good, and I recommend this to others who are doing lyso-IP using a tagged TMEM192.

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

TMEM192 Antibody Western Blot,Immunoprecipitation validation (1:1000 dilution) in HEK293T (Cat no:28263-1-AP)