TMEM120A Polyklonaler Antikörper

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• KD/KO Validated

TMEM120A Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 17455-1-AP

Synonyme

NET29, TACAN, TMEM120A, TMPIT, transmembrane protein 120A

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 17455-1-AP

  WB result of TMEM120A antibody (17455-1-AP; 1:2400; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM120A transfected HEK-293 cells.

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  WB analysis using 17455-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17455-1-AP (TMEM120A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 17455-1-AP

  Immunofluorescent analysis of HeLa cells using 17455-1-AP (TMEM120A antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, Maus-Cerebellum-Gewebe
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IF: See 2 publications below

Produktinformation

17455-1-AP bindet in WB, IF, ELISA TMEM120A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TMEM120A fusion protein Ag10849
• Vollständiger Name: transmembrane protein 120A
• Berechnetes Molekulargewicht: 322 aa, 36 kDa, 41 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC029487
• Gene symbol: TMEM120A
• Gene ID (NCBI): 83862
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TMEM120A is a transmembrane protein. The function of TMEM120A has not been widely studied, and is yet to be fully elucidated.

Publikationen

Species	Application	Title
human	WB,IF	Nat Commun; Gain-of-function genetic screening identifies the antiviral function of TMEM120A via STING activation.; Authors - Shuo Li
rat	WB	Biomedicines; Neurodynamic Treatment Promotes Mechanical Pain Modulation in Sensory Neurons and Nerve Regeneration in Rats.; Authors - Giacomo Carta
rat	WB,IF	Arch Oral Biol; Upregulation of TACAN in the trigeminal ganglion affects pain transduction in acute pulpitis; Authors - Yicong Shang
	WB	J Physiol; Regulation of the PKD2 channel function by TACAN; Authors - Xiong Liu
human,rat	WB	Low Urin Tract Symptoms; The expression and distribution of TACAN in human and rat bladders; Authors - Qudong Lu
mouse	WB	Cell Metab; Bone marrow immune cells respond to fluctuating nutritional stress to constrain weight regain; Authors - Hai-Yan Zhou