TMEM119 Polyklonaler Antikörper

TMEM119 Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27585-1-AP

Synonyme

OBIF, Osteoblast induction factor, TMEM119, transmembrane protein 119, UNQ731/PRO1415



Geprüfte Anwendungen

Erfolgreiche Detektion in IHCMaushirngewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Immunhistochemie (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

27585-1-AP bindet in WB, IHC, IF, FC, ELISA TMEM119 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen TMEM119 fusion protein Ag26269
Vollständiger Name transmembrane protein 119
Berechnetes Molekulargewicht 29 kDa
Beobachtetes Molekulargewicht 45 kDa
GenBank-ZugangsnummerNM_181724
Gene symbol TMEM119
Gene ID (NCBI) 338773
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TMEM119 immunohistochemistry might provide a useful tool for investigating the biology and pathology of human microglia(PMID: 26250788). Microglia can be detected clearly using Catalog#27585-1-AP.

Protokolle

Produktspezifische Protokolle
IHC protocol for TMEM119 antibody 27585-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanIHC

Sci Adv

Single-cell analysis of human basal cell carcinoma reveals novel regulators of tumor growth and the tumor microenvironment.

Authors - Christian F Guerrero-Juarez
mouse,humanFC

Nat Commun

P-selectin axis plays a key role in microglia immunophenotype and glioblastoma progression.

Authors - Eilam Yeini
humanIF

Glia

Transmembrane protein 119 is neither a specific nor a reliable marker for microglia.

Authors - Elise Vankriekelsvenne
mouseIF

Pharmacol Res

MSCs-extracellular vesicles attenuated neuroinflammation, synapse damage and microglial phagocytosis after hypoxia-ischemia injury by preventing osteopontin expression.

Authors - Danqing Xin
mouseIF

Front Immunol

Didymin Suppresses Microglia Pyroptosis and Neuroinflammation Through the Asc/Caspase-1/GSDMD Pathway Following Experimental Intracerebral Hemorrhage.

Authors - Lingui Gu
human

Neurochem Res

BMP7 Attenuates Neuroinflammation after Spinal Cord Injury by Suppressing the Microglia Activation and Inducing Microglial Polarization Via the STAT3 Pathway

Authors - Xiaojin Wei

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Hannes (Verified Customer) (07-27-2020)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)IHC-P image of anti-TMEM119 (27585-1-AP) stained murine and human brain. The tissue was paraffin-embedded, formalin fixed. The tissue was then incubated with EnVision+ Single Reagents (HRP. Rabbit), undiluted for 1 hour at room temperature.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Murine and human brain tissue
TMEM119 Antibody Immunohistochemistry validation (1:500 dilution) in Murine and human brain tissue (Cat no:27585-1-AP)