TMEM102 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TMEM102 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 25505-1-AP

Synonyme

CBAP, TMEM102, transmembrane protein 102

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  WB analysis of HepG2 using 25505-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25505-1-AP (TMEM102 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 25505-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25505-1-AP (TMEM102 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 25505-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25505-1-AP (TMEM102 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 25505-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25505-1-AP (TMEM102 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 25505-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25505-1-AP (TMEM102 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 25505-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25505-1-AP (TMEM102 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human heart using 25505-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25505-1-AP (TMEM102 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 25505-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25505-1-AP (TMEM102 Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen, Jurkat-Zellen, Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

25505-1-AP bindet in WB, IF, IHC, ELISA TMEM102 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TMEM102 fusion protein Ag22213
• Vollständiger Name: transmembrane protein 102
• Berechnetes Molekulargewicht: 508 aa, 54 kDa
• Beobachtetes Molekulargewicht: 50-60 kDa
• GenBank-Zugangsnummer: BC046189
• Gene symbol: TMEM102
• Gene ID (NCBI): 284114
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IF	J Biol Chem; CBAP regulates the function of Akt-associated TSC protein complexes to modulate mTORC1 signaling; Authors - Wei-Ting Liao ; KD Validated