TMP21 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TMP21 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Rezensionen (2)

Kat-Nr. : 15199-1-AP

Synonyme

TMED10, 21 kDa transmembrane-trafficking protein, p23, p24 family protein delta-1, p24delta1

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  WB analysis using 15199-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15199-1-AP (TMP21 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15199-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15199-1-AP (TMP21 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of BxPC-3 using 15199-1-AP

  IP result of anti-TMP21 (IP:15199-1-AP, 4ug; Detection:15199-1-AP 1:3000) with BxPC-3 cells lysate 2080 ug.

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  IHC staining of mouse brain using 15199-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15199-1-AP (TMP21 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15199-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15199-1-AP (TMP21 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15199-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15199-1-AP (TMP21 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15199-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15199-1-AP (TMP21 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15199-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15199-1-AP (TMP21 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 15199-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 15199-1-AP (TMP21 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 15199-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 15199-1-AP (TMP21 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen, HEK-293T-Zellen, Maus-Pankreasgewebe, Neuro-2a-Zellen, NIH/3T3-Zellen, PC-3-Zellen, Raji-Zellen, Ratten-Pankreasgewebe, U-87 MG-Zellen
• Erfolgreiche IP: BxPC-3-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Kolonkarzinomgewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

15199-1-AP bindet in WB, IHC, IF, IP, CoIP, ELISA TMP21 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TMP21 fusion protein Ag7347
• Vollständiger Name: transmembrane emp24-like trafficking protein 10 (yeast)
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC001825
• Gene symbol: TMED10
• Gene ID (NCBI): 10972
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse,human	WB,IF	Cell; A Translocation Pathway for Vesicle-Mediated Unconventional Protein Secretion.; Authors - Min Zhang ; KO Validated
human	WB	Nat Cell Biol; A dual role of ERGIC-localized Rabs in TMED10-mediated unconventional protein secretion; Authors - Yuxin Sun
human	WB	Cell Res; A new type of ERGIC-ERES membrane contact mediated by TMED9 and SEC12 is required for autophagosome biogenesis.; Authors - Shulin Li
human	WB	Brain Behav Immun; Active secretion of IL-33 from astrocytes is dependent on TMED10 and promotes central nervous system homeostasis; Authors - Mengya Jiao
human	CoIP	J Cell Sci; A Wntless-SEC12 complex on the ER membrane regulates early Wnt secretory vesicle assembly and mature ligand export.; Authors - Jiaxin Sun
human	WB,IF	BMC Cancer; The circular RNA CDR1as regulate cell proliferation via TMED2 and TMED10.; Authors - Xue Yang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

pitna (Verified Customer) (12-19-2019)

Tmp21, a subunit of the p24 complexTo reduce non-specific binding, blots were incubated in LiCor Buffer for an hour at room temperature. Representative images from Western blots of total homogenate and ER fractions

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1,000
• Cell Tissue Type: Postmortem human brain : dorsolateral prefrontal cortex (DLPFC)

TMP21 Antibody Western Blot, validation (1:1,000 dilution) in Postmortem human brain : dorsolateral prefrontal cortex (DLPFC) (Cat no:15199-1-AP)

FH

Boyan (Verified Customer) (01-25-2019)

Good. By IF, PFA fixation, it labels the protein at the Golgi.

• Applications: Immunofluorescence,