TK1 Polyklonaler Antikörper

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• KD/KO Validated

TK1 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 15691-1-AP

Synonyme

thymidine kinase 1, soluble, Thymidine kinase, cytosolic, TK1, TK2

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Galerie der Validierungsdaten

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  WB analysis using 15691-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15691-1-AP (TK1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 15691-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15691-1-AP (TK1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 15691-1-AP

  IP result of anti-TK1(IP:15691-1-AP, 4ug; Detection:15691-1-AP 1:800) with HEK-293 cells lysate 1400 ug.

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  IHC staining of human breast cancer using 15691-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15691-1-AP (TK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15691-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15691-1-AP (TK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15691-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15691-1-AP (TK1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 15691-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15691-1-AP (TK1 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of U2OS using 15691-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using TK1 antibody (15691-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, MCF-7-Zellen, SW 1990-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

15691-1-AP bindet in WB, IP, IHC, IF, ELISA TK1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TK1 fusion protein Ag8249
• Vollständiger Name: thymidine kinase 1, soluble
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 25-30 kDa
• GenBank-Zugangsnummer: BC007986
• Gene symbol: TK1
• Gene ID (NCBI): 7083
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TK1(Thymidine kinase, cytosolic) belongs to the thymidine kinase family and is a regulatory enzyme that can interchange between its dimeric and tetrameric forms, which have low and high catalytic efficiencies, respectively. TK1 is involved in DNA synthesis and an important proliferation marker(PMID:21178264).

Publikationen

Species	Application	Title
human,mouse	WB,IHC	Cell Metab; Thymidine kinase 1 drives hepatocellular carcinoma in enzyme-dependent and -independent manners; Authors - Qing Li ; KD Validated
rat	IF	Theranostics; Intravenous transplantation of olfactory ensheathing cells reduces neuroinflammation after spinal cord injury via interleukin-1 receptor antagonist.; Authors - Lijian Zhang
human	WB	Oxid Med Cell Longev; lncRNA IGF2-AS Regulates Nucleotide Metabolism by Mediating HMGA1 to Promote Pyroptosis of Endothelial Progenitor Cells in Sepsis Patients.; Authors - Guibin Liang
mouse,human	WB	Cancer Biol Ther; rAAV9-UPII-TK-EGFP can precisely transduce a suicide gene and inhibit the growth of bladder tumors.; Authors - Foyan Lian
mouse	WB	Adv Biol Regul; Fhit and Wwox loss-associated genome instability: A genome caretaker one-two punch.; Authors - Morgan S Schrock
human	WB	J Cell Mol Med; Down-regulation of TFAM increases the sensitivity of tumour cells to radiation via p53/TIGAR signalling pathway.; Authors - Xu Jiang