TINAG-Specific Polyklonaler Antikörper

TINAG-Specific Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19747-1-AP

Synonyme

TIN AG, TINAG

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Galerie der Validierungsdaten

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  WB analysis of mouse small intestine using 19747-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 19747-1-AP (TINAG-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 19747-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 19747-1-AP (TINAG-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 19747-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 19747-1-AP (TINAG-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 19747-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 19747-1-AP (TINAG-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 19747-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 19747-1-AP (TINAG-Specific antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human colon using 19747-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 19747-1-AP (TINAG-Specific antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Dünndarmgewebe, humanes Nierengewebe, humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

19747-1-AP bindet in WB, IHC,ELISA TINAG-Specific und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: tubulointerstitial nephritis antigen
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: NM_014464
• Gene symbol: TINAG
• Gene ID (NCBI): 27283
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TINAG belongs to the peptidase C1 family. TINAG mediates adhesion of proximal tubule epithelial cells via integrins alpha3-beta1 and alphaV-beta3. It is a non catalytic peptidase C1 family protein. Antibodies against TINAG are found in sera of patients with tubulointerstitial nephritis, a rare autoimmune disorder that causes acute and chronic renal injury.It has 2 isoforms produced by alternative splicing. The antibody is specific to TINAG.