TIMP-2 Polyklonaler Antikörper

TIMP-2 Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

33

Kat-Nr. : 17353-1-AP

Synonyme

CSC 21K, Metalloproteinase inhibitor 2, TIMP 2, TIMP2, TIMP-2

---

Galerie der Validierungsdaten

• 

  IHC staining of human kidney using 17353-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17353-1-AP (TIMP-2 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 17353-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17353-1-AP (TIMP-2 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse lung using 17353-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 17353-1-AP (TIMP-2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 17353-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 17353-1-AP (TIMP-2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 17353-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17353-1-AP (TIMP-2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Prostatakarzinomgewebe, Mauslungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 7 publications below
• IF: See 1 publications below

Produktinformation

17353-1-AP bindet in IHC, IF, ELISA TIMP-2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TIMP-2 fusion protein Ag10051
• Vollständiger Name: TIMP metallopeptidase inhibitor 2
• Berechnetes Molekulargewicht: 220 aa, 24 kDa
• GenBank-Zugangsnummer: BC052605
• Gene symbol: TIMP2
• Gene ID (NCBI): 7077
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The TIMPs are natural highly specific endogenous inhibitors of MMPs. Human TIMP family consists of four 21-28 kDa proteins known as TIMP1, TIMP2, TIMP3, and TIMP4 encoded by four paralogous genes.TIMPs are vital to the maintenance of ECM homeostasis primarily through their MMP inhibitory functions. TIMP2, a member of the TIMP family, regulates the proteolytic activity of all MMPs and is involved in cell differentiation, growth, migration, angiogenesis, and apoptosis. Urinary TIMP2 has been recently recognized as an early biomarker to predict Acute kidney injury (AKI) in critically ill patients.

Publikationen

Species	Application	Title
human	IHC,IF	Clin Cancer Res; RAB37 Hypermethylation Regulates Metastasis and Resistance to Docetaxel-Based Induction Chemotherapy in Nasopharyngeal Carcinoma.; Authors - Yingqin Li
human		Cell Death Dis; α1,3-fucosylation of MEST promotes invasion potential of cytotrophoblast cells by activating translation initiation; Authors - Hao Wang
mouse		Int J Mol Sci; PTEN Inhibitor Treatment Lowers Muscle Plasma Membrane Damage and Enhances Muscle ECM Homeostasis after High-Intensity Eccentric Exercise in Mice; Authors - Baile Wu
human		Int J Biol Sci; TDG suppresses the migration and invasion of human colon cancer cells via the DNMT3A/TIMP2 axis.; Authors - Jiyu Miao
human		Mar Drugs; 11-epi-Sinulariolide Acetate Reduces Cell Migration and Invasion of Human Hepatocellular Carcinoma by Reducing the Activation of ERK1/2, p38MAPK and FAK/PI3K/AKT/mTOR Signaling Pathways.; Authors - Jen-Jie Lin
human	IHC	Cell Signal; Integration of exosomal miR-106a and mesothelial cells facilitates gastric cancer peritoneal dissemination.; Authors - Meng Zhu