TICAM2 Polyklonaler Antikörper

TICAM2 Polyklonal Antikörper für FC, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, FC,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19524-1-AP

Synonyme

TICAM 2, TICAM2, TIRAP3, TIRP, TRAM, TRIF related adapter molecule

---

Galerie der Validierungsdaten

• 

  WB analysis of PC-3 using 19524-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 19524-1-AP (TICAM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 19524-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19524-1-AP (TICAM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 19524-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 19524-1-AP (TICAM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 19524-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19524-1-AP (TICAM2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 19524-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 19524-1-AP (TICAM2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate hyperplasia using 19524-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 19524-1-AP (TICAM2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate hyperplasia using 19524-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 19524-1-AP (TICAM2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of HeLa using 19524-1-AP

  1X10^6 HeLa cells were stained with 0.2ug TICAM2 antibody (19524-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, HeLa-Zellen, humanes Hodengewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Prostatahyperplasie-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

19524-1-AP bindet in WB, IHC, FC,ELISA TICAM2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: toll-like receptor adaptor molecule 2
• Berechnetes Molekulargewicht: 27 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: NM_021649
• Gene symbol: TICAM2
• Gene ID (NCBI): 353376
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TICAM2, also named as TIRAP3, TIRP and TRAM, is involved in IL1-triggered NF-kappa-B activation, functioning upstream of IRAK1, IRAK2, TRAF6, and IKBKB. Physically bridges TLR4 and TICAM1 and functionally transmits LPS-TRL4 signal to TICAM1. TICAM2 is involved in LPS-TLR4 signaling to regulate the MYD88-independent pathway during the innate immune response to LPS.In WB, the MW of TICAM2 is about 27-32kd.