THOC7 Polyklonaler Antikörper

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• KD/KO Validated

THOC7 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 17881-1-AP

Synonyme

fSAP24, hTREX30, NIF3L1 binding protein 1, NIF3L1BP1, THO complex subunit 7 homolog, THOC7

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Galerie der Validierungsdaten

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  WB analysis using 17881-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17881-1-AP (THOC7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 17881-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 17881-1-AP (THOC7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 17881-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17881-1-AP (THOC7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 17881-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17881-1-AP (THOC7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 17881-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17881-1-AP (THOC7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 17881-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17881-1-AP (THOC7 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 17881-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17881-1-AP (THOC7 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 17881-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17881-1-AP (THOC7 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 17881-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17881-1-AP (THOC7 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of A431 using 17881-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using 17881-1-AP (THOC7 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, humanes Lebergewebe, Maushodengewebe, Rattenhodengewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Hodengewebe, humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below

Produktinformation

17881-1-AP bindet in WB, IHC, IF, ELISA THOC7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: THOC7 fusion protein Ag11795
• Vollständiger Name: THO complex 7 homolog (Drosophila)
• Berechnetes Molekulargewicht: 204 aa, 24 kDa
• Beobachtetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: BC065012
• Gene symbol: THOC7
• Gene ID (NCBI): 80145
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

THOC7 is a member of the THO complex that associate with the mRNA export apparatus. It is required for efficient export of polyadenylated RNA. It acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production.

Publikationen

Species	Application	Title
human	WB	Viruses; Murine Leukemia Virus Uses TREX Components for Efficient Nuclear Export of Unspliced Viral Transcripts.; Authors - Toshie Sakuma ; KD Validated