TH1L Monoklonaler Antikörper

TH1L Monoklonal Antikörper für FC, IF, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, FC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1A10G2

Kat-Nr. : 67380-1-Ig

Synonyme

HSPC130, Negative elongation factor C/D, NELF C, NELF C/D, NELF D, NELFD, TH1, TH1 like (Drosophila), TH1 like protein, TH1L

---

Galerie der Validierungsdaten

• 

  WB analysis using 67380-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67380-1-Ig (TH1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis of RAW 264.7 using 67380-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 67380-1-Ig (TH1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 67380-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67380-1-Ig (TH1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 67380-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67380-1-Ig (TH1L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 67380-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67380-1-Ig (TH1L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 67380-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67380-1-Ig (TH1L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 67380-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67380-1-Ig (TH1L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 67380-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 67380-1-Ig (TH1L antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HeLa using 67380-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TH1L antibody (67380-1-Ig, Clone: 1A10G2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HeLa using 67380-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TH1L antibody (67380-1-Ig, Clone: 1A10G2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HeLa using 67380-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TH1L (67380-1-Ig, Clone:1A10G2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, RAW 264.7-Zellen
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:10000
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67380-1-Ig bindet in WB, IF, FC, ELISA TH1L und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TH1L fusion protein Ag24894
• Vollständiger Name: TH1-like (Drosophila)
• Berechnetes Molekulargewicht: 66 kDa
• Beobachtetes Molekulargewicht: 60-66 kDa
• GenBank-Zugangsnummer: BC014952
• Gene symbol: TH1L
• Gene ID (NCBI): 51497
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.