TH Monoklonaler Antikörper

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• KD/KO Validated

TH Monoklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

21

CloneNo.

2H7B7

Kat-Nr. : 66334-1-Ig

Synonyme

TH, TYH, Tyrosine 3 hydroxylase, Tyrosine 3 monooxygenase, tyrosine hydroxylase

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Galerie der Validierungsdaten

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  WB analysis of SH-SY5Y using 66334-1-Ig

  WB result of TH antibody (66334-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TH transfected SH-SY5Y cells.

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  WB analysis of SH-SY5Y using 66334-1-Ig

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66334-1-Ig (TH Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-12 using 66334-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 66334-1-Ig (TH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-12 using 66334-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 66334-1-Ig (TH Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of SH-SY5Y using 66334-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed SH-SY5Y cells using 66334-1-Ig(TH antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-12-Zellen, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IF: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:3000-1:20000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 12 publications below
• IHC: See 6 publications below
• IF: See 7 publications below

Produktinformation

66334-1-Ig bindet in WB, IHC, IF, ELISA TH und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TH fusion protein Ag23075
• Vollständiger Name: tyrosine hydroxylase
• Berechnetes Molekulargewicht: 528 aa, 59 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC104967
• Gene symbol: TH
• Gene ID (NCBI): 7054
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TH(Tyrosine 3-monooxygenase) converts L-tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA), the essential and rate-limiting step to formation of DA and other catecholamines. TH plays an important role in the physiology of adrenergic neurons and can be used as a marker for DA and noradrenergic neurons.

Publikationen

Species	Application	Title
human	WB	Mol Oncol; Norepinephrine-CREB1-miR-373 axis promotes progression of colon cancer.; Authors - Jia Han
	WB	Front Cell Dev Biol; RAB39B Deficiency Impairs Learning and Memory Partially Through Compromising Autophagy.; Authors - Mengxi Niu
human	IF	Antioxidants (Basel); Mitochondrial Calcium-Triggered Oxidative Stress and Developmental Defects in Dopaminergic Neurons Differentiated from Deciduous Teeth-Derived Dental Pulp Stem Cells with MFF Insufficiency; Authors - Xiao Sun
	WB,IF	Bone Joint Res; High-intensity interval training improves fatty infiltration in the rotator cuff through the β3 adrenergic receptor in mice; Authors - Hecheng Zhou
	IHC	Biomed Pharmacother; Nardosinone regulates the slc38a2 gene to alleviate Parkinson's symptoms in rats through the GABAergic synaptic and cAMP pathways.; Authors - Li-Hua Bian
	IHC	J Neuropathol Exp Neurol; Regular Aerobic Exercise-Alleviated Dysregulation of CAMKIIα Carbonylation to Mitigate Parkinsonism via Homeostasis of Apoptosis With Autophagy.; Authors - Wenfeng Liu