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  • KD/KO Validated

TGFBR2 Monoklonaler Antikörper

TGFBR2 Monoklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

CloneNo.

2D5H7

Kat-Nr. : 66636-1-Ig

Synonyme

AAT3, FAA3, LDS1B, LDS2B, MFS2, RIIC, TAAD2, TbetaR II, TGF beta receptor type 2, TGF beta receptor type II, TGF beta type II receptor, TGFbeta RII, TGFBR2, TGFR 2



Geprüfte Anwendungen

Erfolgreiche Detektion in WBA549-Zellen, HEK-293T-Zellen, HepG2-Zellen, K-562-Zellen, L02-Zellen, NCCIT-Zellen, NIH/3T3-Zellen, THP-1-Zellen, U2OS-Zellen
Erfolgreiche Detektion in IHChumanes Mammakarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in FCTHP-1-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:20000
Immunhistochemie (IHC)IHC : 1:250-1:1000
Durchflusszytometrie (FC)FC : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

66636-1-Ig bindet in WB, IP, IHC, IF, FC, CoIP, ELISA TGFBR2 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus, Ratte, Ziege
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen TGFBR2 fusion protein Ag25773
Vollständiger Name transforming growth factor, beta receptor II (70/80kDa)
Berechnetes Molekulargewicht 65 kDa
Beobachtetes Molekulargewicht 65-80 kDa
GenBank-ZugangsnummerBC040499
Gene symbol TGFBR2
Gene ID (NCBI) 7048
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TGF beta Receptor II (TGFBR2) is a member of the transforming growth factor-β (TGF-β) superfamily, which are critical regulators of cell proliferation and differentiation, developmental patterning and morphogenesis, and disease pathogenesis (PMID: 10974075). TGFBR2 result in both SMAD4-dependent constraint of proliferation and SMAD4-independent activation of apoptosis (PMID: 29396446). It has some isoforms with the molecular weight of 70-80 kDa and ~90 kDa after glycosylated.

Protokolle

Produktspezifische Protokolle
WB protocol for TGFBR2 antibody 66636-1-IgProtokoll herunterladen
IHC protocol for TGFBR2 antibody 66636-1-IgProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB

Cell Rep Med

Engineering irradiated tumor-derived microparticles as personalized vaccines to enhance anti-tumor immunity

Authors - Yajie Sun
mouse,ratIF,WB,IHC

Acta Pharmacol Sin

GPR97 deficiency ameliorates renal interstitial fibrosis in mouse hypertensive nephropathy

Authors - Ji-Chao Wu
ratWB,IP

Br J Pharmacol

Inhibition of HSP90 S-nitrosylation Alleviates Cardiac Fibrosis via TGFβ/SMAD3 Signaling Pathway.

Authors - Xiyue Zhang
ratIHC

NPJ Regen Med

Alkaline shear-thinning micro-nanocomposite hydrogels initiate endogenous TGFβ signaling for in situ bone regeneration

Authors - Yuting Niu
human,mouseWB,IHC

Acta Pharmacol Sin

N-n-Butyl haloperidol iodide ameliorates liver fibrosis and hepatic stellate cell activation in mice.

Authors - Dai-Fei Shen
  • KD Validated
humanWB,IP

Aging (Albany NY)

A novel ALG10/TGF-β positive regulatory loop contributes to the stemness of colorectal cancer.

Authors - Xiaotian Xu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Kenzo (Verified Customer) (08-15-2023)

This antibody worked well for labeling TGF beta receptor type 2 in mouse kidney. We were able to acquire robust immunofluorescence signals with this KO validated antibody.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Mouse Kidney tissue