TGFBR2 Polyklonaler Antikörper

TGFBR2 Polyklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 27212-1-AP

Synonyme

AAT3, FAA3, LDS1B, LDS2B, MFS2, RIIC, TAAD2, TbetaR II, TGF beta receptor type 2, TGF beta receptor type II, TGF beta type II receptor, TGFbeta RII, TGFBR2, TGFR 2

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Galerie der Validierungsdaten

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  WB analysis using 27212-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27212-1-AP (TGFBR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 27212-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27212-1-AP (TGFBR2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 27212-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27212-1-AP (TGFBR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of THP-1 using 27212-1-AP

  1X10^6 THP-1 cells were intracellularly stained with 0.4 ug Anti-Human TGFBR2 (27212-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: THP-1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 1 publications below
• FC: See 1 publications below

Produktinformation

27212-1-AP bindet in WB, IHC, FC, ELISA TGFBR2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TGFBR2 fusion protein Ag25767
• Vollständiger Name: transforming growth factor, beta receptor II (70/80kDa)
• Berechnetes Molekulargewicht: 65 kDa
• Beobachtetes Molekulargewicht: 65-80 kDa
• GenBank-Zugangsnummer: BC040499
• Gene symbol: TGFBR2
• Gene ID (NCBI): 7048
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TGF beta Receptor II (TGFBR2) is a member of the transforming growth factor-β (TGF-β) superfamily, which are critical regulators of cell proliferation and differentiation, developmental patterning and morphogenesis, and disease pathogenesis (PMID: 10974075). TGFBR2 result in both SMAD4-dependent constraint of proliferation and SMAD4-independent activation of apoptosis (PMID: 29396446). It has some isoforms with the molecular weight of 70-80 kDa and ~90 kDa after glycosylated.

Publikationen

Species	Application	Title
mouse	FC	Cell Commun Signal; The activation of PPARγ enhances Treg responses through up-regulating CD36/CPT1-mediated fatty acid oxidation and subsequent N-glycan branching of TβRII/IL-2Rα.; Authors - Yumeng Miao
human	WB	Reprod Sci; Maternal Perinatal Exposure to Dibutyl Phthalate Promotes Ovarian Dysfunction in Adult Female Offspring via Downregulation of TGF-β2 and TGF-β3.; Authors - Jing Zhang
mouse	WB	Acta Histochem; Inhibition of glutamine transporter ASCT2 mitigates bleomycin-induced pulmonary fibrosis in mice; Authors - Naqi Lian
human,mouse	WB,IHC	Front Pharmacol; 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside ameliorates bleomycin-induced pulmonary fibrosis via regulating pro-fibrotic signaling pathways; Authors - Tsung-Teng Huang
sheep	WB	Theriogenology; Exploration the role of INHBA in Hu sheep granulosa cells using RNA-Seq; Authors - Yongjin Bao
human	WB	Cell Chem Biol; Therapeutic potency of compound RMY-205 for pulmonary fibrosis induced by SARS-CoV-2 nucleocapsid protein; Authors - Zhi-Yuan Zhang