TGFBI / BIGH3 Monoklonaler Antikörper

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• KD/KO Validated

TGFBI / BIGH3 Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

IF, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

3E11D11

Kat-Nr. : CL594-60007

Synonyme

Beta ig h3, BIGH3, CDB1, CDG2, CDGG1, CSD, CSD1, CSD2, CSD3, EBMD, Kerato epithelin, LCD1, RGD CAP, TGFBI, TGFBI / BIGH3

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Galerie der Validierungsdaten

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  IF Staining of human colon cancer using CL594-60007

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®594 TGFBI / BIGH3 antibody (CL594-60007, Clone: 3E11D11 ) at dilution of 1:200.

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  IF Staining of human colon cancer using CL594-60007

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®594 TGFBI / BIGH3 antibody (CL594-60007, Clone: 3E11D11 ) at dilution of 1:200.

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  FC experiment of Y79 using CL594-60007

  1X10^6 Y79 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human TGFBI / BIGH3 (CL594-60007, Clone:3E11D11) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: humanes Kolonkarzinomgewebe
• Erfolgreiche Detektion in FC: Y79-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-60007 bindet in IF, FC (Intra) TGFBI / BIGH3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TGFBI / BIGH3 fusion protein Ag0241
• Vollständiger Name: transforming growth factor, beta-induced, 68kDa
• Berechnetes Molekulargewicht: 683 aa, 75 kDa
• GenBank-Zugangsnummer: BC000097
• Gene symbol: TGFBI
• Gene ID (NCBI): 7045
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TGFBI, also named as BIGH3, Kerato-epithelin and RGD-CAP, binds to type I, II, and IV collagens. TGFBI is an adhesion protein which may play an important role in cell-collagen interactions. In cartilage, it may be involved in endochondral bone formation. TGFBI is an extracellular matrix adaptor protein, it has been reported to be differentially expressed in transformed tissues. TGFBI is a predictive factor of the response to chemotherapy, and suggest the use of TGFBI-derived peptides as possible therapeutic adjuvants for the enhancement of responses to chemotherapy.(PMID:20509890) Defects in TGFBI are the cause of epithelial basement membrane corneal dystrophy (EBMD). Defects in TGFBI are the cause of corneal dystrophy Groenouw type 1 (CDGG1). Defects in TGFBI are the cause of corneal dystrophy lattice type 1 (CDL1). Defects in TGFBI are a cause of corneal dystrophy Thiel-Behnke type (CDTB). Defects in TGFBI are the cause of Reis-Buecklers corneal dystrophy (CDRB). Defects in TGFBI are the cause of lattice corneal dystrophy type 3A (CDL3A). Defects in TGFBI are the cause of Avellino corneal dystrophy (ACD).

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C