TGF Beta 1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TGF Beta 1 Polyklonal Antikörper für FC, IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (4)

Anwendung

WB, IP, IHC, IF, FC, CoIP, Cell treatment, ELISA

Konjugation

Unkonjugiert

Publikationen

529

Rezensionen (7)

Kat-Nr. : 21898-1-AP

Synonyme

CED, DPD1, LAP, Mouse Transforming Growth Factor β1, TGF beta 1, tgf beta1, TGFB, TGFB1, TGFbeta, TGF-beta 1, tgfbeta1, tgf-beta1, TGFβ, TGF-β, TGFβ1, TGF-β1

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Galerie der Validierungsdaten

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  WB analysis using 21898-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21898-1-AP (TGF-beta 1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 21898-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 21898-1-AP (TGF Beta 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 21898-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 21898-1-AP (TGF-beta 1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21898-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21898-1-AP (TGF-beta 1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 21898-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 21898-1-AP (TGF-beta 1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skin using 21898-1-AP

  IP result of anti-TGF Beta 1(IP:21898-1-AP, 4ug; Detection:21898-1-AP 1:6000) with mouse skin tissue lysate 640 ug.

• 

  IF Staining of HEK-293 using 21898-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using TGF Beta 1 antibody (21898-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of HEK-293 using 21898-1-AP

  1X10^6 HEK-293 cells were intracellularly stained with 0.25 ug Anti-Human TGF Beta 1 (21898-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.25 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushautgewebe, A549-Zellen, HeLa-Zellen, K-562-Zellen, Rattenhautgewebe
• Erfolgreiche IP: Maushautgewebe
• Erfolgreiche Detektion in IF: HEK-293-Zellen
• Erfolgreiche Detektion in FC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:200-1:800
• Durchflusszytometrie (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 416 publications below
• IHC: See 121 publications below
• IF: See 53 publications below
• IP: See 1 publications below
• ELISA: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

21898-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, Cell treatment, ELISA TGF Beta 1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Hund, Kaninchen, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TGF Beta 1 fusion protein Ag13591
• Vollständiger Name: transforming growth factor, beta 1
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 44 kDa
• GenBank-Zugangsnummer: BC000125
• Gene symbol: TGFB1
• Gene ID (NCBI): 7040
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TGFB, also named as LAP and TGFB1, is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. TGFB acts synergistically with TGFA in inducing transformation. It also acts as a negative autocrine growth factor. Dysregulation of TGFB activation and signaling may result in apoptosis. Many cells synthesize TGFB and almost all of them have specific receptors for it. TGFB positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. It is highly expressed in bone. Mutation of TGFB are the cause of Camurati-Engelmann disease (CED) which known as progressive diaphyseal dysplasia 1 (DPD1).

This antibody detects the pro-TGF beta 1 and the cleaved fragment Latency-associated peptide.

Publikationen

Species	Application	Title
	IHC	Adv Mater; Neonatal Tissue-derived Extracellular Vesicle Therapy (NEXT): A Potent Strategy for Precision Regenerative Medicine; Authors - Peng Lou
human	WB	ACS Nano; Mesenchymal Stem Cell-Derived Extracellular Vesicles Attenuate Mitochondrial Damage and Inflammation by Stabilizing Mitochondrial DNA.; Authors - Meng Zhao
human	WB	Blood; C1Q labels a highly aggressive macrophage-like leukemia population indicating extramedullary infiltration and relapse; Authors - Li-Xue Yang
	WB,IF	Acta Pharm Sin B; Fucoidan-functionalized activated platelet-hitchhiking micelles simultaneously track tumor cells and remodel the immunosuppressive microenvironment for efficient metastatic cancer treatment.; Authors - Rong Guo
human	WB	J Exp Clin Cancer Res; ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK; Authors - Li Cui
		Autophagy; Trehalose induces autophagy via lysosomal-mediated TFEB activation in models of motoneuron degeneration.; Authors - Paola Rusmini

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kenzo (Verified Customer) (08-15-2023)

This antibody works well for labeling TGF beta 1 on mouse kidney tissue section. Very reliable antibody giving reproducible results.

• Applications: Immunofluorescence
• Cell Tissue Type: Mouse Kidney

FH

Celina (Verified Customer) (07-31-2023)

antibody worked well for IF of human cardiac ventricular fibroblasts

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: human cardiac ventricular fibroblasts

FH

Udesh (Verified Customer) (12-06-2022)

The antibody worked well in Western Blot and detected bands for mature TGF-B1 as well as LAP.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

TGF Beta 1 Antibody Western Blot validation (1:1000 dilution) in (Cat no:21898-1-AP)

FH

Yasuyo (Verified Customer) (05-24-2022)

Works great!!!

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: lung

TGF Beta 1 Antibody Immunohistochemistry validation (1:250 dilution) in lung (Cat no:21898-1-AP)

FH

P (Verified Customer) (12-01-2021)

Excellent

• Applications: Western Blot
• Cell Tissue Type: fibroblast

TGF Beta 1 Antibody Western Blot validation ( dilution) in fibroblast (Cat no:21898-1-AP)

FH

Iram (Verified Customer) (09-04-2020)

Very clean bands for western

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000 for western
• Cell Tissue Type: Human colon cancer

FH

Qingyuan (Verified Customer) (11-05-2019)

The antibody clearly shows one band with the right molecular weight size.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse spinal cord

Verwandte Produkte

Flow Cytometry Perm Buffer (10X)

PF00011-C