Thyroglobulin Monoklonaler Antikörper

Thyroglobulin Monoklonal Antikörper für IF, IHC, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

CloneNo.

4D3C5

Kat-Nr. : 60272-1-Ig

Synonyme

AITD3, TG, TGN, thyroglobulin

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Galerie der Validierungsdaten

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  IHC staining of human thyroid using 60272-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid tissue slide using 60272-1-Ig (Thyroglobulin antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human thyroid using 60272-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid tissue slide using 60272-1-Ig (Thyroglobulin antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human thyroid cancer using 60272-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60272-1-Ig (Thyroglobulin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 60272-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60272-1-Ig (Thyroglobulin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 60272-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60272-1-Ig (Thyroglobulin antibody at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human thyroid cancer using 60272-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60272-1-Ig (Thyroglobulin antibody at dilution of 1:100 (under 40x lens).

• 

  IF Staining of human thyroid cancer using 60272-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human thyroid cancer tissue using Thyroglobulin antibody (60272-1-Ig, Clone: 4D3C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human thyroid cancer using 60272-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human thyroid cancer tissue using Thyroglobulin antibody (60272-1-Ig, Clone: 4D3C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Schilddrüsengewebe, humanes Schilddrüsenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Schilddrüsenkarzinomgewebe

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

60272-1-Ig bindet in IHC, IF, ELISA Thyroglobulin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Thyroglobulin fusion protein Ag18070
• Vollständiger Name: thyroglobulin
• Berechnetes Molekulargewicht: 2768 aa, 305 kDa
• GenBank-Zugangsnummer: BC140933
• Gene symbol: TG
• Gene ID (NCBI): 7038
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Thyroglobulin (Tg) is a glycoprotein homodimer produced predominantly by the thryroid gland. It acts as a substrate for the synthesis of thyroxine and triiodothyronine as well as the storage of the inactive forms of thyroid hormone and iodine. Thyroglobulin is secreted from the endoplasmic reticulum to its site of iodination, and subsequent thyroxine biosynthesis, in the follicular lumen. Mutations in this gene cause thyroid dyshormonogenesis, manifested as goiter, and are associated with moderate to severe congenital hypothyroidism. Polymorphisms in this gene are associated with susceptibility to autoimmune thyroid diseases (AITD) such as Graves disease and Hashimoto thryoiditis.

Publikationen

Species	Application	Title
human	IF	Front Immunol; Proteomics and Organoid Culture Reveal the Underlying Pathogenesis of Hashimoto's Thyroiditis.; Authors - Hui Xiao
human	IHC	Cancer Cell Int; Identification of an immune-related signature indicating the dedifferentiation of thyroid cells.; Authors - Xuemin Wang