Trefoil factor 3 Polyklonaler Antikörper

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• KD/KO Validated

Trefoil factor 3 Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Rezensionen (1)

Kat-Nr. : 23277-1-AP

Synonyme

HITF, hP1.B, Intestinal trefoil factor, ITF, Polypeptide P1.B, TFF3, TFI, Trefoil factor 3, trefoil factor 3 (intestinal)

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Galerie der Validierungsdaten

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  IHC staining of human colon using 23277-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 23277-1-AP (Trefoil factor 3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 23277-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 23277-1-AP (Trefoil factor 3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 23277-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon slide using 23277-1-AP (Trefoil factor 3 antibody at dilution of 1:50.

• 

  IHC staining of human colon using 23277-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon slide using 23277-1-AP (Trefoil factor 3 antibody at dilution of 1:50.

• 

  IHC staining of human small intestine using 23277-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 23277-1-AP (Trefoil factor 3 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human small intestine using 23277-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 23277-1-AP (Trefoil factor 3 antibody at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below

Produktinformation

23277-1-AP bindet in WB, IHC, ELISA Trefoil factor 3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Trefoil factor 3 fusion protein Ag9543
• Vollständiger Name: trefoil factor 3 (intestinal)
• Berechnetes Molekulargewicht: 80 aa, 9 kDa
• GenBank-Zugangsnummer: BC017859
• Gene symbol: TFF3
• Gene ID (NCBI): 7033
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Trefoil factor 3 (TFF3) belongs to the TFF-domain peptide family which consists of three small secreted proteins (TFF1, TFF2, TFF3) that are expressed by mucous-secreting epithelia. TFF3 is a major constituent in the goblet cells in small and large intestines, especially a typical secretary peptide of the normal human antral and pyloric gastric mucosa. TFF3 is essential in regulating cell migration and maintaining normal GI mucosal integrity. TFF3 has been reported to be overexpressed at the gene and the protein level in human neoplasms such as prostate, breast, and colon cancer. TFF3 is involved in tumor cell scattering, angiogenesis, and invasion.

Publikationen

Species	Application	Title
human	WB	EMBO J; The chromatin remodeler SRCAP promotes self-renewal of intestinal stem cells.; Authors - Buqing Ye
	WB,IHC	Neoplasia; Downregulation of Notch Signaling in Kras-Induced Gastric Metaplasia.; Authors - Wen-Cheng Chung
human	IHC	Rhinology; Expression of Trefoil factor family peptides in the nasal allergic mucosa.; Authors - Miyahara Nobuyuki N
human	WB	J Histotechnol; Trefoil factor 3 knock-down prevents autophagy-related gene 12 elevation in colon adenocarcinoma.; Authors - Zhuo Zhang ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Iru (Verified Customer) (06-21-2019)

There blot doesn't come out clean.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Fallopian tube epithelial cells

Trefoil factor 3 Antibody Western Blot, validation (1:1000 dilution) in Fallopian tube epithelial cells (Cat no:23277-1-AP)