TFE3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TFE3 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Kat-Nr. : 14480-1-AP

Synonyme

bHLHe33, RCCP2, TFE3, TFEA, Transcription factor E3

---

Galerie der Validierungsdaten

• 

  WB analysis of K-562 using 14480-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14480-1-AP (TFE3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 14480-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14480-1-AP (TFE3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 14480-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14480-1-AP (TFE3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 14480-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 14480-1-AP (TFE3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 14480-1-AP

  IP Result of anti-TFE3 (IP:14480-1-AP, 4ug; Detection:14480-1-AP 1:500) with K-562 cells lysate 1200ug.

• 

  IHC staining of human renal cell carcinoma using 14480-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 14480-1-AP (TFE3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 14480-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 14480-1-AP (TFE3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, humanes Hirngewebe, Mausmilzgewebe, Rattenhirngewebe
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierenzellkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 12 publications below
• IF: See 5 publications below
• IP: See 2 publications below
• ChIP: See 1 publications below

Produktinformation

14480-1-AP bindet in WB, IP, IHC, IF, ChIP, ELISA TFE3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TFE3 fusion protein Ag5863
• Vollständiger Name: transcription factor binding to IGHM enhancer 3
• Berechnetes Molekulargewicht: 62 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC026027
• Gene symbol: TFE3
• Gene ID (NCBI): 7030
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.

Publikationen

Species	Application	Title
human	IF	Cell Death Differ; Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration; Authors - Huaizhen Liang
human	WB	Autophagy; Oxidation of multiple MiT/TFE transcription factors links oxidative stress to transcriptional control of autophagy and lysosome biogenesis.; Authors - Hongfeng Wang
human	WB,IF,IP	Autophagy; PRCC-TFE3 fusion-mediated PRKN/parkin-dependent mitophagy promotes cell survival and proliferation in PRCC-TFE3 translocation renal cell carcinoma.; Authors - Bo Wang ; KD Validated
mouse	WB	Cell Discov; Targeting macrophage TFEB-14-3-3 epsilon Interface by naringenin inhibits abdominal aortic aneurysm.; Authors - Yiting Jia
human	WB,IF	Bioorg Med Chem; Terpenoids isolated from Chinese liverworts Lepidozia reptans and their anti-inflammatory activity.; Authors - Siwen Li
human	WB	Biochim Biophys Acta Gen Subj; Anti-cancer effect of marchantin C via inducing lung cancer cellular senescence associated with less secretory phenotype.; Authors - Xiu-Lei Zhang