Transferrin Rekombinanter Antikörper

Transferrin Rekombinant Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

5O7

Kat-Nr. : 81676-1-RR

Synonyme

Beta 1 metal binding globulin, DKFZp781D0156, PRO1557, PRO2086, Serotransferrin, Siderophilin, TF, transferrin

---

Galerie der Validierungsdaten

• 

  WB analysis using 81676-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81676-1-RR (Transferrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 81676-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81676-1-RR (Transferrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human hepatocirrhosis using 81676-1-RR

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 81676-1-RR (Transferrin antibody) at dilution of 1:950 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 81676-1-RR

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 81676-1-RR (Transferrin antibody) at dilution of 1:950 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: human plasma, mouse serum, rat serum, human urine
• Erfolgreiche Detektion in IHC: humanes Leberzirrhosegewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:475-1:1900

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

81676-1-RR bindet in WB, IHC, ELISA Transferrin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Transferrin fusion protein Ag11504
• Vollständiger Name: transferrin
• Berechnetes Molekulargewicht: 698 aa, 77 kDa
• Beobachtetes Molekulargewicht: 77 kDa
• GenBank-Zugangsnummer: BC059367
• Gene symbol: TF
• Gene ID (NCBI): 7018
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Serotransferrin(TF) is a 77 kDa secreted protein which is also named as transferrin, siderophilin, beta-1 metal-binding globulin and belongs to a family of homologous iron-binding glycoproteins that encompasses lactoferrin (found both intracellular and in secretions, including milk), melanotrasferrin (present on melanoma cells) and ovotransferrin (present in egg white). TF is a multi-function protein with a primary role in transporting iron in a safe, redox-inactive state from absorption to utilization or storage sites around the body. The association of Tf with the immune system derives from its ability to restrict serum free-iron levels, creating low-iron environments where the infection capacity of pathogenic microorganisms is limited. TF is expressed predominantly in the liver and secreted in plasma, but lower amounts can be synthesized in other tissues such as the brain and the testis.