TEX264 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TEX264 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 25858-1-AP

Synonyme

SIG11, testis expressed 264, TEX264, ZSIG11

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Galerie der Validierungsdaten

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  WB analysis using 25858-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25858-1-AP (TEX264 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 25858-1-AP

  WB result of TEX264 antibody (25858-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TEX264 transfected HEK-293 cells.

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  WB analysis of mouse testis using 25858-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25858-1-AP (TEX264 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 25858-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25858-1-AP (TEX264 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 25858-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25858-1-AP (TEX264 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse testis using 25858-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25858-1-AP (TEX264 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 25858-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25858-1-AP (TEX264 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 25858-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25858-1-AP (TEX264 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Maushirngewebe, Maushodengewebe, Neuro-2a-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IF: See 3 publications below

Produktinformation

25858-1-AP bindet in WB, IHC, IF, ELISA TEX264 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TEX264 fusion protein Ag23027
• Vollständiger Name: testis expressed 264
• Berechnetes Molekulargewicht: 313 aa, 34 kDa
• Beobachtetes Molekulargewicht: 37 kDa
• GenBank-Zugangsnummer: BC008742
• Gene symbol: TEX264
• Gene ID (NCBI): 51368
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TEX264 (testes expressed gene 264) is a single-pass transmembrane protein, consisting of an N-terminal hydrophobic region, a gyrase inhibitory (GyrI)-like domain, and a loosely structured C terminus. TEX264 was first identified as an endoplasmic reticulum (ER)-resident Atg8-family-binding protein that mediates the degradation of portions of the ER during starvation (i.e., reticulophagy). TEX264 was identified as a cofactor of VCP/p97 ATPase that promotes the repair of covalently trapped TOP1 (DNA topoisomerase 1)-DNA crosslinks.

Publikationen

Species	Application	Title
human	IF	Autophagy; ATF4 links ER stress with reticulophagy in glioblastoma cells.; Authors - Svenja Zielke
mouse	WB,IF	ACS Chem Neurosci; Anti-Inflammatory Anthranilate Analogue Enhances Autophagy through mTOR and Promotes ER-Turnover through TEX264 during Alzheimer-Associated Neuroinflammation.; Authors - Zhiyu Wang ; KD Validated
mouse	WB	Biomedicines; Enhancement of TEX264-Mediated ER-Phagy Contributes to the Therapeutic Effect of Glycycoumarin against APA Hepatotoxicity in Mice.; Authors - Mingzhu Yan
human	WB,IF	Mol Biol Cell; Lysosome Docking to WIPI1 Rings and ER-connected Phagophores Occurs During DNAJB12 and GABARAP-Dependent Selective Autophagy of Misfolded P23H-Rhodopsin.; Authors - Andrew Kennedy
human	WB	Mol Biol Cell; CCPG1 recognizes ER luminal proteins for selective ER-phagy; Authors - Shunsuke Ishii
human	WB	Food Chem Toxicol; Excessive ER-phagy contributes to ochratoxin A-induced apoptosis; Authors - Huiqiong Deng