TEX14 Polyklonaler Antikörper

TEX14 Polyklonal Antikörper für IHC, IP,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18351-1-AP

Synonyme

SGK307, SGK307, Sugen kinase 307, testis expressed 14, Testis expressed protein 14, Testis expressed sequence 14, TEX14



Geprüfte Anwendungen

Erfolgreiche Detektion in IHCMaushodengewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Immunhistochemie (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

18351-1-AP bindet in WB, IHC, IF, ELISA TEX14 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen TEX14 fusion protein Ag13177
Vollständiger Name testis expressed 14
Berechnetes Molekulargewicht957aa,107 kDa; 1497aa,168 kDa
Beobachtetes Molekulargewicht 180-200 kDa, 106 kDa
GenBank-ZugangsnummerBC040526
Gene symbol TEX14
Gene ID (NCBI) 56155
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tex14 is required both for the formation of intercellular bridges during meiosis and for kinetochore-microtubule attachment during mitosis. Intercellular bridges are evolutionarily conserved structures that connect differentiating germ cells and are required for spermatogenesis and male fertility. Tex14 acts by promoting the conversion of midbodies into intercellular bridges via its interaction with CEP55: interaction with CEP55 inhibits the interaction between CEP55 and PDCD6IP/ALIX and TSG101, blocking cell abscission and leading to transform midbodies into intercellular bridges.Tex14 also plays a role during mitosis: recruited to kinetochores by PLK1 during early mitosis and regulates the maturation of the outer kinetochores and microtubule attachment. Tex14 has several variant isoforms with the MW from about 100 kDa to 168 kDa.

Protokolle

Produktspezifische Protokolle
WB protocol for TEX14 antibody 18351-1-APProtokoll herunterladen
IHC protocol for TEX14 antibody 18351-1-APProtokoll herunterladen
IP protocol for TEX14 antibody 18351-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB,IF

Int J Mol Sci

MCF7 Spheroid Development: New Insight about Spatio/Temporal Arrangements of TNTs, Amyloid Fibrils, Cell Connections, and Cellular Bridges.

Authors - Laura Pulze
human,mouseIF

FASEB J

Dazl determines primordial follicle formation through the translational regulation of Tex14.

Authors - Roseanne Rosario
mouseIHC

Methods Mol Biol

Whole-mount immunohistochemistry to study spermatogonial stem cells and spermatogenic lineage development in mice, monkeys, and humans.

Authors - Kathrin Gassei
mouseIF

Cell Death Dis

Specific deletion of protein phosphatase 6 catalytic subunit in Sertoli cells leads to disruption of spermatogenesis.

Authors - Wen-Long Lei
mouseIF

Cells

The Male Mouse Meiotic Cilium Emanates from the Mother Centriole at Zygotene Prior to Centrosome Duplication

Authors - Pablo López-Jiménez