TEX10 Polyklonaler Antikörper

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• KD/KO Validated

TEX10 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 17372-1-AP

Synonyme

Nbla10363, testis expressed 10, TEX10

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 17372-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17372-1-AP (TEX10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17372-1-AP

  WB result of TEX10 antibody (17372-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-TEX10 transfected HeLa cells.

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  IHC staining of human brain using 17372-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17372-1-AP (TEX10 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human brain using 17372-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17372-1-AP (TEX10 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 17372-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17372-1-AP (TEX10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 10 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Produktinformation

17372-1-AP bindet in WB, IP, IHC, IF, ELISA TEX10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TEX10 fusion protein Ag11014
• Vollständiger Name: testis expressed 10
• Berechnetes Molekulargewicht: 929 aa, 106 kDa
• Beobachtetes Molekulargewicht: 95-100 kDa
• GenBank-Zugangsnummer: BC030652
• Gene symbol: TEX10
• Gene ID (NCBI): 54881
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TEX10 is a component of some MLL1/MLL complex, at least composed of the core components MLL, ASH2L, HCFC1/HCF1, WDR5 and RBBP5. PELP1-TEX10-WDR18 complex is a regulator of ribosome biogenesis and suggests that its SUMO-controlled distribution coordinates the rate of ribosome formation. And this complex is required for the maturation and nucleolar release of the large ribosomal subunit. (PMID:21326211)

Publikationen

Species	Application	Title
human	WB,IF	EMBO J; The SUMO system controls nucleolar partitioning of a novel mammalian ribosome biogenesis complex.; Authors - Finkbeiner Elisabeth E ; KD Validated
human	WB	Mol Cell Proteomics; Five friends of methylated chromatin target of protein-arginine-methyltransferase[prmt]-1 (chtop), a complex linking arginine methylation to desumoylation.; Authors - Fanis Pavlos P
human	WB,IHC,IF	Cell Cycle; Tex10 is upregulated and promotes cancer stem cell properties and chemoresistance in hepatocellular carcinoma.; Authors - Xiaocong Xiang ; KD Validated
human	WB,IF	Mol Biol Cell; LAS1L interacts with the mammalian Rix1 complex to regulate ribosome biogenesis.; Authors - Castle Christopher D CD
human	WB	J Immunol Res; TEX10 Promotes the Tumorigenesis and Radiotherapy Resistance of Urinary Bladder Carcinoma by Stabilizing XRCC6; Authors - Sheng Luo ; KD Validated
human	WB,IHC	Oncol Rep; Tex10 promotes stemness and EMT phenotypes in esophageal squamous cell carcinoma via the Wnt/β‑catenin pathway.; Authors - Xiaocong Xiang ; KD Validated