TET2 Polyklonaler Antikörper

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• KD/KO Validated

TET2 Polyklonal Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

Kat-Nr. : CL594-21207

Synonyme

FLJ20032, KIAA1546, tet oncogene family member 2, TET2

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Galerie der Validierungsdaten

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  FC experiment of HeLa using CL594-21207

  1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human TET2 (CL594-21207) (red), or 0.8 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Durchflusszytometrie (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-21207 bindet in FC (Intra) TET2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TET2 fusion protein Ag15583
• Vollständiger Name: tet oncogene family member 2
• Berechnetes Molekulargewicht: 2002 aa, 224 kDa
• Beobachtetes Molekulargewicht: 130 kDa, 133 kDa, 224 kDa
• GenBank-Zugangsnummer: BC150180
• Gene symbol: TET2
• Gene ID (NCBI): 54790
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TET2 is an enzyme which converts 5-methylcytosine (5-mC) to 5-hydroxymethylcytosine (5-hmC) in DNA and is important for normal myelopoiesis. TET2 mutations are frequently observed in myeloid malignancies, including myeloproliferative neoplasms. TET2 is broadly expressed in a variety of tissues. In healthy tissues, TET2 expression was shown to be elevated in hematopoietic cells with highest expression in granulocytes. Three isoforms of TET2 exists due to the alternative splicing. 220-250 kDa bands represent the full-length isoform; 130-133 kDa bands represent the nuclear isoforms of TET2. (PMID: 24615693) This antibody recognizes all three isoforms of TET2.

Verwandte Produkte

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011