TERF2IP Polyklonaler Antikörper

TERF2IP Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 14595-1-AP

Synonyme

DRIP5, hRap1, RAP1 homolog, TERF2IP

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Galerie der Validierungsdaten

• 

  WB analysis of HEK-293 using 14595-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14595-1-AP (TERF2IP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 14595-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14595-1-AP (TERF2IP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14595-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14595-1-AP (TERF2IP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 14595-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14595-1-AP (TERF2IP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 14595-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 14595-1-AP (TERF2IP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 14595-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 14595-1-AP (TERF2IP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14595-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14595-1-AP (TERF2IP antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 14595-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 14595-1-AP (TERF2IP antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 14595-1-AP

  IP Result of anti-TERF2IP (IP:14595-1-AP, 3ug; Detection:14595-1-AP 1:800) with HeLa cells lysate 2720ug.

• 

  IHC staining of human tonsillitis using 14595-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14595-1-AP (TERF2IP Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human tonsillitis using 14595-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14595-1-AP (TERF2IP Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HeLa using 14595-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TERF2IP antibody (14595-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  FC experiment of HeLa using 14595-1-AP

  1X10^6 HeLa cells were stained with 0.2ug TERF2IP antibody (14595-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A431-Zellen, HeLa-Zellen, HT-1080.Zellen, humanes Plazenta-Gewebe, K-562-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

14595-1-AP bindet in WB, IP, IHC, IF, FC, ELISA TERF2IP und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TERF2IP fusion protein Ag6130
• Vollständiger Name: telomeric repeat binding factor 2, interacting protein
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC005841
• Gene symbol: TERF2IP
• Gene ID (NCBI): 54386
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TERF2IP, also named as DRIP5, RAP1, is a 399 amino acid protein, which belongs to the RAP1 family. TERF2IP associates with chromosomes, both at telomeres and in extratelomeric sites. TERF2IP acts both as a regulator of telomere function and as a transcription regulator. TERF2IP is involved in the regulation of telomere length and protection as a component of the shelterin complex (telosome). In contrast to other components of the shelterin complex, it is dispensible for telomere capping and does not participate in the protection of telomeres against non-homologous end-joining (NHEJ)-mediated repair. The calculated molecular weight of TERF2IP is 44 kDa, but modified TERF2IP, is about 60 kDa. (PMID: 15838871)

Publikationen

Species	Application	Title
human	WB	Nucleic Acids Res; Preferential extension of short telomeres induced by low extracellular pH.; Authors - Yuanlong Ge
human	WB	Exp Cell Res; C9ORF78 partially localizes to centromeres and plays a role in chromosome segregation.; Authors - Radhika Koranne