TERF2 Polyklonaler Antikörper

TERF2 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 22020-1-AP

Synonyme

Telomeric DNA binding protein, TERF2, TRBF2, TRF2, TTAGGG repeat binding factor 2

---

Galerie der Validierungsdaten

• 

  WB analysis of Jurkat using 22020-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22020-1-AP (TERF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 22020-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22020-1-AP (TERF2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 22020-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 22020-1-AP (TERF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 22020-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22020-1-AP (TERF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 22020-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 22020-1-AP (TERF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 22020-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22020-1-AP (TERF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 22020-1-AP

  IP Result of anti-TERF2 (IP:22020-1-AP, 4ug; Detection:22020-1-AP 1:500) with K-562 cells lysate 4000ug.

• 

  IHC staining of human gliomas using 22020-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 22020-1-AP (TERF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 22020-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 22020-1-AP (TERF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 22020-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 22020-1-AP (TERF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 22020-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 22020-1-AP (TERF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 22020-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22020-1-AP (TERF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 22020-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22020-1-AP (TERF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, MCF-7-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, Maushirngewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below

Produktinformation

22020-1-AP bindet in WB, IP, IHC, ELISA TERF2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TERF2 fusion protein Ag16798
• Vollständiger Name: telomeric repeat binding factor 2
• Berechnetes Molekulargewicht: 56 kDa
• Beobachtetes Molekulargewicht: 65-69 kDa
• GenBank-Zugangsnummer: BC024890
• Gene symbol: TERF2
• Gene ID (NCBI): 7014
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRF2, also named as TRBF2 and Telomeric repeat-binding factor 2, is a 542 amino acid protein, which contains 1 HTH myb-type DNA-binding domain and localizes in the Nucleus. TRF2 binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. TRF2 is a component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. TRF2 together with DCLRE1B/Apollo, is required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B), which is needed for telomere replication during fork passage and prevent aberrant telomere topology. TRF2 recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length.

Publikationen

Species	Application	Title
human	WB	Oxid Med Cell Longev; Inhibition of TRF2 Leads to Ferroptosis, Autophagic Death, and Apoptosis by Causing Telomere Dysfunction; Authors - Qiuhui Yang ; KD Validated
human	WB	Cell Mol Biol Lett; MCCC2 is a novel mediator between mitochondria and telomere and functions as an oncogene in colorectal cancer; Authors - Wanjun Liu