TER-119 Monoklonaler Antikörper

TER-119 Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2b

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

APC Fluorescent Dye

CloneNo.

TER-119

Kat-Nr. : APC-65149

Synonyme

Ly76, lymphocyte antigen 76, TER 119, Ter119, TER-119

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Galerie der Validierungsdaten

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  FC experiment of mouse bone marrow cells using APC-65149

  1X10^6 C57BL/6 mouse bone marrow cells were surface stained with FITC-Anti-Mouse CD45 (FITC-65087, Clone: 30-F11) and 0.50 ug APC Anti-Mouse TER-119 (APC-65149, Clone: TER-119). Cells were not fixed.

• 

  FC experiment of mouse bone marrow cells using APC-65149

  1X10^6 C57BL/6 mouse bone marrow cells were surface stained with FITC-Anti-Mouse CD45 (FITC-65087, Clone: 30-F11) and 0.50 ug APC-Rat IgG2b isotype control antibody. Cells were not fixed.

• 

  FC experiment of mouse bone marrow cells using APC-65149

  1X10^6 C57BL/6 mouse bone marrow cells were surface stained with 0.20 ug APC-Anti-Mouse TER-119 (APC-65149, clone TER-119) (red) or 0.20 ug APC-Rat IgG2b isotype control (blue). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Knochenmarkzellen

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

APC-65149 bindet in FC TER-119 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Fetale Leberzellen von Tag 14 aus einer C57BL/6-Maus
• Vollständiger Name: lymphocyte antigen 76
• Gene symbol: Ly76
• Gene ID (NCBI): 104231
• Konjugation: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: Phosphatbasierter Puffer mit 0,09% Natriumazid und 0,1% Gelatine, pH 7,2.
• Lagerungsbedingungen: Bei 2-8°C lagern. Vor Licht schützen.

Hintergrundinformationen

TER-119 is a mouse erythroid lineage-specific monoclonal antibody that reacts with erythroid cells at differentiation stages from early proerythroblast to mature erythrocyte, but not with cells showing typical erythroid blast-forming unit (BFU-E) and erythroid colony-forming unit (CFU-E) activities (PMID: 1975515; 10848813). TER-119 recognizes a 52-kDa molecule on erythrocyte membranes (PMID: 10848813). TER-119 antigen is a molecule associated with cell-surface glycophorin A but not with glycophorin A itself (PMID: 10848813).