TEAD3 Monoklonaler Antikörper

TEAD3 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

2E6A12

Kat-Nr. : 68464-1-Ig

Synonyme

DTEF 1, ETFR 1, TEA domain family member 3, TEAD 3, TEAD3, TEAD5, TEF 5, TEF5

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Galerie der Validierungsdaten

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  WB analysis using 68464-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68464-1-Ig (TEAD3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68464-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68464-1-Ig (TEAD3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HCT 116-Zellen, Hausschwein-Herzgewebe, HepG2-Zellen, JAR-Zellen, U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68464-1-Ig bindet in WB, ELISA TEAD3 und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: TEAD3 fusion protein Ag4407
• Vollständiger Name: TEA domain family member 3
• Berechnetes Molekulargewicht: 435 aa, 49 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC027877
• Gene symbol: TEAD3
• Gene ID (NCBI): 7005
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TEAD3, others named transcription enhancer factor 5(TEF5), is a member of the family of transcription factors that plays a key role in the Hippo signaling pathway, suggesting involvement in organ and tumor suppression by restricting proliferation and promoting. TEAD3 contains the TEA/ATTS DNA-binding domain. Human TEAD3 has a strong mRNA expression in the placenta and choriocarcinoma cells(JEG-3) but is low in Hela, HepG2, and MCF-7.