TDP-43 (for IF/FC) Rekombinanter Antikörper

TDP-43 (for IF/FC) Rekombinant Antikörper für FC (Intra), IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

16A22

Kat-Nr. : CL488-80002

Synonyme

ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43, TDP-43 (for IF/FC)

---

Galerie der Validierungsdaten

• 

  IF Staining of mouse brain using CL488-80002

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL488-80002 (TDP-43 (for IF/FC) antibody) at dilution of 1:100.

• 

  IF Staining of mouse brain using CL488-80002

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL488-80002 (TDP-43 (for IF/FC) antibody) at dilution of 1:100.

• 

  IF Staining of mouse brain using CL488-80002

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL488-80002 (TDP-43 (for IF/FC) antibody) at dilution of 1:100.

• 

  FC experiment of HeLa using CL488-80002

  1X10^6 HeLa cells were stained with 0.2 ug CoraLite® Plus 488 Anti-Human TDP-43 (for IF/FC) (CL488-80002, Clone:16A22) (red), or 0.2 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: Maushirngewebe
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-80002 bindet in IF, FC (Intra) TDP-43 (for IF/FC) und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: TDP-43 (for IF/FC) fusion protein Ag1231
• Vollständiger Name: TAR DNA binding protein
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC001487
• Gene symbol: TARDBP
• Gene ID (NCBI): 23435
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The TARDBP gene encodes the TDP-43 protein, initially found to repress HIV-1 transcription by binding TAR DNA. TDP-43 has since been shown to bind RNA as well as DNA, and have multiple functions in transcriptional repression, translational regulation and pre-mRNA splicing. For instance, it is reported to regulate alternate splicing of the CTFR gene. In 2006 Neumann et al. found that hyperphosphorylated, ubiquitinated and/or cleaved forms of TDP-43, collectively known as pathological TDP-43, play a major role in the disease mechanisms of ubiquitin-positive, tau- and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in amyotrophic lateral sclerosis (ALS).

Verwandte Produkte

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011