TDP-43 (C-terminal) Monoklonaler Antikörper

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• KD/KO Validated

TDP-43 (C-terminal) Monoklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

CloneNo.

1G5F6

Kat-Nr. : 67345-1-Ig

Synonyme

ALS10, TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43, tdp-43, TDP-43 (C-terminal)

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 67345-1-Ig

  WB result of TDP-43 (C-terminal) antibody (67345-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TDP-43 (C-terminal) transfected HeLa cells.

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  WB analysis using 67345-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67345-1-Ig (TDP-43 (C-terminal) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 67345-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67345-1-Ig (TDP-43 (C-terminal) antibody) at dilution of 1:5000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 67345-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67345-1-Ig (TDP-43 (C-terminal) antibody) at dilution of 1:5000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 67345-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67345-1-Ig (TDP-43 (C-terminal) antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat brain using 67345-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 67345-1-Ig (TDP-43 (C-terminal) antibody) at dilution of 1:500 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HeLa using 67345-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.20ug Anti-Human TDP-43 (C-terminal) (67345-1-Ig, Clone:1G5F6) (red) or 0.20 ug Mouse IgG2a Isotype Control(66360-2-Ig, CloneK11A1B2A2) (blue) and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000. Fixed with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, Neuro-2a-Zellen, PC-12-Zellen, SH-SY5Y-Zellen, Y79-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Gliomgewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Rattenhirngewebe
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:2000-1:20000
• Immunfluoreszenz (IF): IF : 1:250-1:1000
• Durchflusszytometrie (FC): FC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 4 publications below

Produktinformation

67345-1-Ig bindet in WB, IHC, IF, FC, ELISA TDP-43 (C-terminal) und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: TAR DNA binding protein
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 43-45 kDa
• GenBank-Zugangsnummer: NM_007375
• Gene symbol: TARDBP
• Gene ID (NCBI): 23435
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Transactivation response (TAR), DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43), was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). 67345-1-Ig is a mouse monoclonal antibody raised against the C-terminal region of human TDP-43.

Publikationen

Species	Application	Title
human	WB,IF	Biomedicines; TDP-43 Pathology and Prionic Behavior in Human Cellular Models of Alzheimer's Disease Patients.; Authors - Eva P Cuevas
human	IF	Neurochem Int; PARP-1 activation leads to cytosolic accumulation of TDP-43 in neurons.; Authors - Joshua M Marcus
human,mouse	WB,IF	ASN Neuro; Casein Kinase 2 Mediates HIV- and Opioid-Induced Pathologic Phosphorylation of TAR DNA Binding Protein 43 in the Basal Ganglia; Authors - Michael Ohene-Nyako
human	WB,IF	Front Mol Neurosci; TTBK1 and CK1 inhibitors restore TDP-43 pathology and avoid disease propagation in lymphoblast from Alzheimer's disease patients; Authors - Loreto Martinez-Gonzalez